JCRB1514 POLK KO homo MSH-
Cell information
Important Notice(s)Cell type：genetically-modified cells (View Pricing Information)
| JCRB No. | JCRB1514 | Cell Name | POLK KO homo MSH- |
|---|---|---|---|
| Profile | Human cell line derived from B cell leukemia. (MSH-, POLK KO/KO, TK+/+) | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 19-year-old |
| Identity | available | Tissue for Primary Cancer | |
| Case history | acute lymphoblastic leukemia (ALL) | Metastasis | |
| Tissue Metastasized | Genetics | MSH-, POLK KO/KO, TK+/+ | |
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | |
| Classify | transformed | Established by | Nohmi, T. |
| Registered by | Nohmi, T. | Regulation for Distribution | restricted |
| Comment | Year | 2012 | |
| Medium | RPMI 1640 + 10% heat-inactivate FBS | Methods for Passages | dilution |
| Cell Number on Passage | twice/week, split ratio = 1:10 | Race | |
| CO2 Conc. | 5% | Tissue Sampling | peripheral blood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:24461735 | In vivo evidence that phenylalanine 171 acts as a molecular brake for translesion DNA synthesis across benzo[a]pyrene DNA adducts by human DNA polymerase κ. Sassa A,Suzuki T,Kanemaru Y,Niimi N,Fujimoto H,Katafuchi A,Grúz P,Yasui M,Gupta RC,Johnson F,Ohta T,Honma M,Adachi N,Nohmi T DNA Repair (Amst). 2014 Mar;15():21-8 |
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LOT Information
| Cell No. | JCRB1514 | Cell Name | POLK KO homo MSH- |
|---|---|---|---|
| LOT No. | 02122013 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | Split ratio 1/10 | Methods for Passages | 0.27-4.2x10^5 cells/ml |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.0x10^6 |
| Viability at cell freezing (%) | 96.1 | Antibiotics Used | free |
| Passage Number | p5* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:9,13 D7S820:8,12 D16S539:10,11 VWA:15,16 TH01:8,9 AM:X,Y TPOX:8,10 CSF1PO:14 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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