JCRB1523 POLK CD MSH-

Cell information

Important Notice(s)

本細胞株の分譲に際しての寄託者の承諾について (On the distribution of this cell line. An approval of depositor is required.)

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1523 Cell Name   POLK CD MSH-
Profile   Human cell line derived from B cell leukemia. (MSH-, POLK catalytically dead, TK+/+) Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   19-year-old
Identity   available Tissue for Primary Cancer   
Case history   acute lymphoblastic leukemia (ALL) Metastasis   
Tissue Metastasized    Genetics   MSH-, POLK catalytically dead (=D198A/D199A), TK+/+
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   transformed Established by   Nohmi, T.
Registered by   Nohmi, T. Regulation for Distribution   Required depositor's approval.
Comment    Year   2012
Medium   RPMI 1640 + 10% heat-inactivate FBS Methods for Passages   dilution
Cell Number on Passage   twice/week, split ratio = 1:10 Race   
CO2 Conc.   5% Tissue Sampling   peripheral blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JH02
Reference
Pubmed id:26031400Catalytic and non-catalytic roles of DNA polymerase κ in the protection of human cells against genotoxic stresses.
Kanemaru Y,Suzuki T,Niimi N,Grúz P,Matsumoto K,Adachi N,Honma M,Nohmi T
Environ Mol Mutagen. 2015 Oct;56(8):650-62

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LOT Information

Cell No.   JCRB1523 Cell Name   POLK CD MSH-
LOT No.   03062013 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.72-2.9x10^5 cells/ml Methods for Passages   Split ratio 1/10
Doubling Time   NT Cell Number in Vial (cells/1ml)   7.2x10^6
Viability at cell freezing (%)   97.6 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:9,14
D7S820:8,12
D16S539:10,11
VWA:15,16
TH01:8,9
AM:X,Y
TPOX:8,10
CSF1PO:14
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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