JCRB1533 293 TRE/Cont

Cell information

Cell type:genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1533 Cell Name   293 TRE/Cont
Profile   Control 293 cell line for 293 TRE/FLAG-POLK. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   embryo
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   G418 resistance
Classify   transformed Established by   Kanemaru, Y.
Registered by   Nohmi, T. Regulation for Distribution   restricted
Comment    Year   2012
Medium   Eagles minimal essential medium with 10% heat inactivated FBS. Methods for Passages   0.25% Trypsin & 1mM EDTA
Cell Number on Passage   1:5-7 Race   
CO2 Conc.   5% Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W25

LOT Information

Cell No.   JCRB1533 Cell Name   293 TRE/Cont
LOT No.   04062013 Lot Specification   distribution
Medium   Eagle's minimum essential medium(Nissui) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   NT Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.Split (1/5-1/7)
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.34x10^6
Viability at cell freezing (%)   100 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information