JCRB1534 IKK-i-DEF

Cell information

Cell type：genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1534 Cell Name   IKK-i-DEF
Profile   Mouse embryonic fibroblasts prepared from IkB kinase (IKK-i) deficient mice. Other Name   
Animal   mouse Strain   C57BL/6
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   Mouse embryonic fibroblasts prepared from IkB kinase (IKK-i) deficient mice.
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   primary Established by   Takeuchi, O.
Registered by   Takeuchi, O. Regulation for Distribution   
Comment    Year   2011
Medium   DMEM 10% FBS Methods for Passages   
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   embryo
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W56
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LOT Information

Cell No.   JCRB1534 Cell Name   IKK-i-DEF
LOT No.   04092013 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.83-4.2x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.12x10^6
Viability at cell freezing (%)   93.0 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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