JCRB1539 HAC-2

Cell information

Important Notice(s)

Difference of two HAC-2 strains, JCRB1359 and JCRB1539

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1539 Cell Name   HAC-2
Profile   Human ovary cancer cell line derived from mesonephros. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   
Identity   available Tissue for Primary Cancer   ovary
Case history   ovarian clear cell adenocarcinoma Metastasis   Yes
Tissue Metastasized    Genetics   Ovarian clear cell adenocarcinoma, tetraploid.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Clear cell carcinoma. Td = 46.8 hrs, CA-125 positive, M type LDH, PAS, Alcian blue positive, mirror ball pattern observed.
Classify   tumor Established by   Nishida,M. & Kuramoto,H.
Registered by   Nishida,M. Regulation for Distribution   
Comment    Year   2013
Medium   Eagle's minimum essential medium with 15% fetal calf serum. Methods for Passages   Cells are harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Cell Number on Passage   1-2 10^5 cells/ml Race   
CO2 Conc.   5% Tissue Sampling   ascites
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_8354
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LOT Information

Cell No.   JCRB1539 Cell Name   HAC-2
LOT No.   08122013 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% heat inactivated fetal bovine serum(SIGMA027K03911,non HI) Temperature   37 C
Cell Density at Seeding   2.7-8.4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   80.8 Antibiotics Used   
Passage Number   p13* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
D13S317:8,9
D7S820:9,11
D16S539:10,11
VWA:14,16
TH01:7
AM:X
TPOX:11
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   Mirror ball pattern is observed.Mycoplasma was eliminated with MC-210.
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Cell No.   JCRB1539 Cell Name   HAC-2
LOT No.   10192017 Lot Specification   distribution
Medium   Eagle's minimum essential medium with 15% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.8 - 5.6 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.1% trypsin and 0.02% EDTA.
Doubling Time   approx. 53 hrs. Cell Number in Vial (cells/1ml)   1.1 x 10^6
Viability at cell freezing (%)   70.0 Antibiotics Used   free
Passage Number   Unknown (15 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - EMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   70.0
Additional information   
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