JCRB1543 PL551Ar

Cell information

Important Notice(s)


On the distribution of human non-immortalized somatic stem cells to oversea users (ethical ragulation).

Cell type:primary mesenchymal stem cell (View Pricing Information)

JCRB No.   JCRB1543 Cell Name   PL551Ar
Profile   Human cell derived from placental artery. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   placenta
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   finite Crisis PDL   
Morphology   epithelial-like Character   
Classify   primary Established by   Umezawa, A.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2011
Medium   EGM-2MV (Lonza) with 5% FBS. Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells.
Cell Number on Passage   0.5-1.0 x 10^6 cells/10cm dish Race   
CO2 Conc.   5% Tissue Sampling   placental artery
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_D692

LOT Information

Cell No.   JCRB1543 Cell Name   PL551Ar
LOT No.   07012013 Lot Specification   distribution
Medium   EGM-2MV BulletKit (LONZA#CC-3202) on collagen I coated dish Temperature   37 C
Cell Density at Seeding   1.0-5.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.025% trypsin and 0.01% EDTA.(1/2-1/4 split)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.90x10^6
Viability at cell freezing (%)   98.0 Antibiotics Used   free
Passage Number   p17 PDL   9.7
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information