JCRB1559 MDA-MB-231-Luc

Cell information

Important Notice(s)

Leibovitz's L-15 medium

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type：luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1559 Cell Name   MDA-MB-231-Luc
Profile   Luciferase stably expressing cell line (MDA-MB-231:Human Breast Cancer). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   51-year-old
Identity   available Tissue for Primary Cancer   mammary gland
Case history   adenocarcinoma Metastasis   Yes
Tissue Metastasized   pleural effusion Genetics   pLVSIN-luc (promoter: CMV) transfected MDA-MB-231 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   puromycin-resistant
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2009
Medium   L-15 medium + 2mM Glutamine + 15% Fetal Bovine Serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/3 - 1/6 Race   Caucasian
CO2 Conc.   - Tissue Sampling   Isolated from pleural effusions of
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG53
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LOT Information

Cell No.   JCRB1559 Cell Name   MDA-MB-231-Luc
LOT No.   10292013 Lot Specification   distribution
Medium   L-15 medium with 15% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding    Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/3-1/6)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.41x10^6
Viability at cell freezing (%)   97.3 Antibiotics Used   
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:13
D7S820:8,9
D16S539:12
VWA:15,18
TH01:7,9.3
AM:X
TPOX:8,9
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   0%
Viability immediately after thawing (%)    Additional information   
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Cell No.   JCRB1559 Cell Name   MDA-MB-231-Luc
LOT No.   09212017 Lot Specification   distribution
Medium   Leivobitz's L-15 medium (GIBCO) with 15% heat inactivated fetal bovine serum (SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.91-2.9x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/3-1/6)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.0x10^6
Viability at cell freezing (%)   99.10 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:13
D7S820:8,9
D16S539:12
VWA:15,18
TH01:7,9.3
AM:X
TPOX:8,9
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   -
Viability immediately after thawing (%)   92 Additional information   
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Cell No.   JCRB1559 Cell Name   MDA-MB-231-Luc
LOT No.   11282017 Lot Specification   distribution
Medium   Leivobitz's L-15 medium (GIBCO) with 15% heat inactivated fetal bovine serum (SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.67-3.4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split ratio=1/3-1/6)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   99.70 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:13
D7S820:8,9
D16S539:12
VWA:15,18
TH01:7,9.3
AM:X
TPOX:8,9
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   0%
Viability immediately after thawing (%)   91.6 Additional information   
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Cell No.   JCRB1559 Cell Name   MDA-MB-231-Luc
LOT No.   04142021 Lot Specification   distribution
Medium   Leivobitz's L-15 medium (GIBCO:cat.No 1145-064) with 15% heat inactivated fetal bovine serum (Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   0.84-1.1x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.53x10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
D13S317:13
D7S820:8,9
D16S539:12
VWA:15,18
TH01:7,9.3
AM:X
TPOX:8,9
CSF1PO:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   0%
Viability immediately after thawing (%)   95.2 Additional information   
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