JCRB1564 TMNK-1

Cell information

Important Notice(s)

JCRB1564 TMNK-1細胞の培養上の注意

This cell line is available for only academic users.

本細胞は学術機関へのみの分譲となります

Endothelial marker expressions in TMNK-1 cells.

Articles regarding TMNK-1 / TWNT-1 / MMNK-1

About the cultivation of TMNK-1 cells.

Cell type:genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1564 Cell Name   TMNK-1
Profile   Immortalized human-liver endothelial cell line with SV40T and hTERT. (Note: recommended medium is very specific.) Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   Immortalized by SV40T and hTERT.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   Expressed factor VIII, VEGF receptors (flt-1, KDR/Flk-1), and CD34.
Classify   transformed Established by   Kobayashi, N.
Registered by   Mochitate, K. Regulation for Distribution   [Matsumura et al., Transplantation 77: 1357-1365, 2004] should be cited at the paper submission.
Comment   Distribution restricted to academic Japan domestic user, or only academic oversea users. The following reference should be cited at the publication of paper. Matsumura et al. Transplantation 77: 1357-1365, 2004. Year   2013
Medium   Mochitate modified MCDB-131 medium with 50% of EGM-2 SingleQuot Kit (clontics #CC-4176; ascorbic acid was substituted by ascorbic acid phosphate magnesium salt n-Hydrate (0.2 mM)) Methods for Passages   Cells were harvested after treatment with accutase (Innovative cell technologies) and split ratio=1/3-1/4
Cell Number on Passage   0.3-1.5x10^5 cells/sq.cm (spli Race   
CO2 Conc.   5% Tissue Sampling   liver
Tissue Type   liver endothelial cell

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W79
Reference
Pubmed id:15167590 Establishment of an immortalized human-liver endothelial cell line with SV40T and hTERT.
Matsumura T,Takesue M,Westerman KA,Okitsu T,Sakaguchi M,Fukazawa T,Totsugawa T,Noguchi H,Yamamoto S,Stolz DB,Tanaka N,Leboulch P,Kobayashi N
Transplantation. 2004 May 15;77(9):1357-65

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LOT Information

Cell No.   JCRB1564 Cell Name   TMNK-1
LOT No.   03042014 Lot Specification   distribution
Medium   Mochitate modified MCDB(Nissui) with 50% of EGM-2 SingleQuot Kit(Clonetics #CC-4176) Temperature   37 C
Cell Density at Seeding   0.3-1.5x10^5 cells/sq.cm Methods for Passages   Cells were harvested after treatment with accutase(Innovative cell technologies) and split ratio=1/3-1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.13x10^6
Viability at cell freezing (%)   93.9 Antibiotics Used   
Passage Number   p20 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:11,13
D16S539:10,11
VWA:15,16
TH01:6,7
AM:X
TPOX:9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Mochitate modified MCDB with 100% of EGM-2 SingleQuot Kit and 10% DMSO CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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Cell No.   JCRB1564 Cell Name   TMNK-1
LOT No.   04132018 Lot Specification   distribution
Medium   Mochitate modified MCDB131(PEPTIDE Institute Inc.;custom order) with 50% of EGM-2 SingleQuot Kit(Clonetics #CC-4176) Temperature   37 C
Cell Density at Seeding   2-4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with accutase (Innovative cell technologies) and split ratio=1/3-1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.09x10^6
Viability at cell freezing (%)   95.7 Antibiotics Used   free
Passage Number   p22 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:11,13
D16S539:10,11
VWA:15,16
TH01:6,7
AM:X
TPOX:9
CSF1PO:10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Mochitate modified MCDB131 with 100% of EGM-2 SingleQuot Kit and 10% DMSO CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   Mycoplasma elimination by MC-210 treatment. 1vial is 0.5ml.
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