JCRB1568 KKU-100
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1568 | Cell Name | KKU-100 |
|---|---|---|---|
| Profile | Human poorly differentiated cholangiocarcinoma cell line established from biliary tract. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 65-year-old |
| Identity | available | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | poorly differentiated cholangiocarcinoma |
| Classify | tumor | Established by | Spira, B. |
| Registered by | Okada, S. | Regulation for Distribution | Acknowledgment is requested |
| Comment | In publishing the research results to be obtained by use of the cell lines, an acknowledgment to the Dr. Banchob Spira (Khon Kaen University) is requested. | Year | 2013 |
| Medium | DMEM with 10% Fetal bovine serum. | Methods for Passages | Cells harvested after treatment with trypsin and EDTA. |
| Cell Number on Passage | ? | Race | |
| CO2 Conc. | 5% | Tissue Sampling | biliary tract |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:15948244 | Establishment and characterization of an opisthorchiasis-associated cholangiocarcinoma cell line (KKU-100). Sripa B,Leungwattanawanit S,Nitta T,Wongkham C,Bhudhisawasdi V,Puapairoj A,Sripa C,Miwa M World J Gastroenterol. 2005 Jun 14;11(22):3392-7 |
| Images |
|---|
      |
| Movies |
|---|
|
LOT Information
| Cell No. | JCRB1568 | Cell Name | KKU-100 |
|---|---|---|---|
| LOT No. | 01312014 | Lot Specification | distribution |
| Medium | DMEM with 10 % heat inactivated fetal bovine serum(SIGMA027K03911) | Temperature | 37 C |
| Cell Density at Seeding | 0.78-3.33x10^4cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.13x10^6 |
| Viability at cell freezing (%) | 92.1 | Antibiotics Used | free |
| Passage Number | p9* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,13 D13S317:9 D7S820:8,11 D16S539:10,11 VWA:17,19 TH01:8,9 AM:X TPOX:8,9 CSF1PO:11,13 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
| Images |
|---|
|
|
| Cell No. | JCRB1568 | Cell Name | KKU-100 |
|---|---|---|---|
| LOT No. | 07122016 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) | Temperature | 37 C |
| Cell Density at Seeding | 3.4 - 7.3 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | approx. 33 hrs. | Cell Number in Vial (cells/1ml) | 2.4 x 10^6 |
| Viability at cell freezing (%) | 98.4 | Antibiotics Used | free |
| Passage Number | Unknown (11 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 98.4 | Additional information |
| Cell No. | JCRB1568 | Cell Name | KKU-100 |
|---|---|---|---|
| LOT No. | 04012024 | Lot Specification | distribution |
| Medium | Dulbecco's modified Eagle's medium with 10% FBS (FBS, Nichirei Cat. # 174112, Lot 20M00K) | Temperature | 37 C |
| Cell Density at Seeding | 2.5 - 6.2 x 10^4 cells/mL | Methods for Passages | Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA. |
| Doubling Time | 29 - 39 hrs. | Cell Number in Vial (cells/1ml) | 6.9 x 10^5 |
| Viability at cell freezing (%) | 100 | Antibiotics Used | free |
| Passage Number | Unknown (11 at bank) | PDL | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | |
| Chromosome Mode | Chromosome Information | ||
| Surface Antigen | DNA Profile (STR) | ||
| Adhesion | Yes | Exoteric Gene | |
| Medium for Freezing | 10% DMSO, 20% FBS - DMEM | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 98.4 | Additional information |