JCRB1574 RSV-M

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1574 Cell Name   RSV-M
Profile   Mouse cell line derived from mouse brain tumor. Other Name   
Animal   mouse Strain   C3H/He
Genus   Mus Species   musclus
Sex   - Age   -
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   SR-RSV-induced brain tumor cells
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   tumor Established by   Sekiguchi, K.
Registered by   Kumanishi, T. Regulation for Distribution   
Comment    Year   2013
Medium   DMEM with 10% FBS Methods for Passages   0.02% Trypsin + 0.01% EDTA
Cell Number on Passage   1:3 - 1:5 /week Race   
CO2 Conc.    Tissue Sampling   brain
Tissue Type   brain tumor

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4Y24
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LOT Information

Cell No.   JCRB1574 Cell Name   RSV-M
LOT No.   02192014 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.13-1.4x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.02% trypsin and 0.01% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.9x10^5
Viability at cell freezing (%)   95.7 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   We recommend to keep cell density under 1.4x10^5 cells/sq.cm to avoid cell detachment from culture dish.
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Cell No.   JCRB1574 Cell Name   RSV-M
LOT No.   10022017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine seru (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   2.6 - 3.8 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.02% trypsin and 0.02% EDTA.
Doubling Time   approx. 15 hrs. Cell Number in Vial (cells/1ml)   4.2 x 10^6
Viability at cell freezing (%)   70.0 Antibiotics Used   free
Passage Number   Unknown (8 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
Viability immediately after thawing (%)   70.0 Additional information   
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