Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1580 Cell Name   SR-CDF1 DBT
Profile   Mouse cell line derived from mouse brain tumor. Other Name   DBT
Animal   mouse Strain   (Balb/c x DBA/2) F1
Genus   Mus Species   musclus
Sex   F Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   SR-RSV-induced brain tumor cells
Life Span   infinite Crisis PDL   
Morphology   bipolar cell Character   sensitive to mouse coronavirus infection
Classify   tumor Established by   Yamamoto, T.
Registered by   Kumanishi, T. Regulation for Distribution   
Comment    Year   2013
Medium   DMEM with 10% FBS Methods for Passages   0.02% Trypsin + 0.01% EDTA
Cell Number on Passage   1:3, 1 or 2 times/week Race   
CO2 Conc.    Tissue Sampling   brain
Tissue Type   brain tumor

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W75
Pubmed id:4301953 Brain tumors induced with Rous sarcoma virus, Schmidt-Ruppin strain. I. Induction of brain tumors in adult mice with Rous chicken sarcoma cells.
Kumanishi T
Jpn J Exp Med. 1967 Oct;37(5):461-74


LOT Information

Cell No.   JCRB1580 Cell Name   SR-CDF1 DBT
LOT No.   04082014 Lot Specification   distribution
Medium   DMEM with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   0.43-1.8x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.02% trypsin and 0.01% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.4x10^6
Viability at cell freezing (%)   75.8 Antibiotics Used   free
Passage Number   p18 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information