JCRB1582 TWNT-1

Cell information

Important Notice(s)

This cell line is available for only academic users.

本細胞は学術機関へのみの分譲となります

Articles regarding TMNK-1 / TWNT-1 / MMNK-1

Cell type:genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1582 Cell Name   TWNT-1
Profile   Immortalized human hepatic stellate cell line (established from LI90). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   available Tissue for Primary Cancer   liver
Case history    Metastasis   No
Tissue Metastasized    Genetics   Expressing catalytic subunit of human telomerase reverse transcriptase (hTERT) and enhanced green fluorescent protein (EGFP).
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   transformed Established by   Kobayashi, N.
Registered by   Mochitate, K. Regulation for Distribution   [Murakami et al., Lab Invest. 72: 731-739, 1995] should be cited at the paper submission.
Comment   The distribution is limited to academic Japan domestic users and academic oversea users. The following reference should be cited at the publication of papers. Murakami et al. Lab Invest. 72: 731-739, 1995. Year   2013
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum. Methods for Passages   0.05% trypsin and 0.53 mM EDTA. split ratio = 1/3 - 1/4.
Cell Number on Passage   1.5 - 6.0 x 10^4 cells/sq.cm Race   
CO2 Conc.   5% Tissue Sampling   LI90 cell line
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_J364
Reference
Pubmed id:12811248 Establishment of immortalized human hepatic stellate scavenger cells to develop bioartificial livers.
Watanabe T,Shibata N,Westerman KA,Okitsu T,Allain JE,Sakaguchi M,Totsugawa T,Maruyama M,Matsumura T,Noguchi H,Yamamoto S,Hikida M,Ohmori A,Reth M,Weber A,Tanaka N,Leboulch P,Kobayashi N
Transplantation. 2003 Jun 15;75(11):1873-80
Pubmed id:7540235 Establishment of a new human cell line, LI90, exhibiting characteristics of hepatic Ito (fat-storing) cells.
Murakami K,Abe T,Miyazawa M,Yamaguchi M,Masuda T,Matsuura T,Nagamori S,Takeuchi K,Abe K,Kyogoku M
Lab Invest. 1995 Jun;72(6):731-9

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LOT Information

Cell No.   JCRB1582 Cell Name   TWNT-1
LOT No.   04082014 Lot Specification   distribution
Medium   DMEM with 10% fetal bovine serum(SIGMA12J396,non HI) Temperature   37 C
Cell Density at Seeding   1.5-6.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.53mM EDTA.Split ratio=1/2-1/4
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.37x10^6
Viability at cell freezing (%)   95.3 Antibiotics Used   
Passage Number   p16 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:12
D7S820:10,11
D16S539:9,10
VWA:16,17
TH01:9
AM:X
TPOX:8,11
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
Cell No.   JCRB1582 Cell Name   TWNT-1
LOT No.   10272017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   approx. 3 x 10^4 cells/mL Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 32 hrs. Cell Number in Vial (cells/1ml)   1.6 x 10^6
Viability at cell freezing (%)   86.6 Antibiotics Used   free
Passage Number   P18 PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   86.6
Additional information   
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