JCRB1584 TYUC-1-Luc

Cell information

Important Notice(s)

本細胞株の民間企業への分譲について

本細胞株の使用について

Announcement of unavailability of this cell line to overseas researcher outside of Japan

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1584 Cell Name   TYUC-1-Luc
Profile   Luciferase stably expressing cell line. Human esophageal adenocarcinoma cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   56-year-old
Identity   available Tissue for Primary Cancer   esophagus
Case history    Metastasis   
Tissue Metastasized    Genetics   pLVSIN-luc transfected TYUC-1 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   floating Character   undifferentiated carcinoma, T4M1N0 stage 3. Puromycin-resistant.
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   not available for oversea researchers.
Comment    Year   2014
Medium   Ham's F12 medium + DMEM (1 to 1 mix) with 5% fetal calf serum. Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   mouse xenograft
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus tested
HCV - HTLV-1 -
HIV-1 - HTLV-2 -
HIV-2 - HAV -
-/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG96
Reference
Pubmed id:25667451 MicroRNA profiles to predict postoperative prognosis in patients with small cell carcinoma of the esophagus.
Okumura T,Shimada Y,Omura T,Hirano K,Nagata T,Tsukada K
Anticancer Res. 2015 Feb;35(2):719-27

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LOT Information

Cell No.   JCRB1584 Cell Name   TYUC-1-Luc
LOT No.   05232014 Lot Specification   distribution
Medium   DMEM and Ham's F12 medium(SiGMA)(1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   3.08-3.89x10^5 cells/ml Methods for Passages   Dilution(Centrifugage at 100-300xg for 3-5 minites to remove dead cells)
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.52x10^6
Viability at cell freezing (%)   86.5 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:8
D7S820:10
D16S539:11
VWA:16
TH01:9
AM:X
TPOX:8
CSF1PO:11
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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