JCRB1586 HSC-5-Luc

Cell information

Important Notice(s)

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines


Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1586 Cell Name   HSC-5-Luc
Profile   Luciferase stably expressing cell line. A cell line derived from human skin squamous cell carcinoma having anaplastic epithelioid features. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   75-year-old
Identity   available Tissue for Primary Cancer   skin
Case history   ulcerated tumor on the upper part of the right auricle. Metastasis   
Tissue Metastasized    Genetics   pLVSIN-luc transfected cells. The cells express No.19 polypeptide{5064}. Modal number of chromosome is reported to be 76
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   The cells lost their ability to differentiate to keratinize. Puromycin-resistant.
Classify   tumor Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2014
Medium   Iscove's modified Dulbecco's medium containing 10% fetal bovine serum. Methods for Passages   Cells were harvested after treatment with 0.02% EDTA and 0.05% trypsin for three minutes. Subculture cells every 2 weeks.
Cell Number on Passage    Race   Japanese
CO2 Conc.   5% Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus tested
HCV - HTLV-1 -
HIV-1 - HTLV-2 -
HIV-2 - HAV -
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG50

LOT Information

Cell No.   JCRB1586 Cell Name   HSC-5-Luc
LOT No.   05302014 Lot Specification   distribution
Medium   IMDM (GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA027K03911) Temperature   37 C
Cell Density at Seeding   1.16-4.53x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.05% trypsin and 0.02% EDTA
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.22x10^6
Viability at cell freezing (%)   98.5 Antibiotics Used   free
Passage Number   p6* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information