Cell information

Important Notice(s)


On the MTA of this cell line with Kyoto University (for profit organizations in Japan)


Announcement of unavailability of this cell line to overseas researcher outside of Japan

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1590 Cell Name   TYGBK-8
Profile   Human gall bladder cancer cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   38-year-old
Identity   available Tissue for Primary Cancer   gallbladder
Case history   gallbladder cancer (pancreaticobiliary maljunction) Metastasis   
Tissue Metastasized    Genetics   p53 mutation exon 7 codon 237 (ATG to ATA?, no K-Ras mutation in codon 12 or 13
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   moderately differentiated tubular carcinoma, T2N1M1, stage4b
Classify   tumor Established by   Shimada, Y.
Registered by   Shimada, Y. Regulation for Distribution   
Comment    Year   2014
Medium   Ham's F12 medium + RPMI1640 (1 to 1 mix) with 5% fetal calf serum (cultued by collagen coat dish). Methods for Passages   Cells harvested after treatment with trypsin and EDTA.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   gall bladder
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_4W81

LOT Information

Cell No.   JCRB1590 Cell Name   TYGBK-8
LOT No.   06272014 Lot Specification   distribution
Medium   RPMI1640 and Ham's F12 medium (1/1) with 5% heat inactivated fetal bovine serum(SIGMA027K03911)and using BioCoat Collagen 1 100mm Dish(Becton Dickinson #354450 or #356450) Temperature   37 C
Cell Density at Seeding   1.33-1.58x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.23x10^6
Viability at cell freezing (%)   97.2 Antibiotics Used   
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information