JCRB1605 Onda 11
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1605 | Cell Name | Onda 11 |
|---|---|---|---|
| Profile | Human cell line derived from human glioma. | Other Name | no.11 |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 64-year-old |
| Identity | available | Tissue for Primary Cancer | brain |
| Case history | glioblastoma | Metastasis | No |
| Tissue Metastasized | Genetics | P53 mutation, PTEN mutation | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | GFAP (glial fibrillary acidic protein positive) |
| Classify | tumor | Established by | Onada, K. |
| Registered by | Kumanishi, T. | Regulation for Distribution | |
| Comment | Year | 2013 | |
| Medium | DMEM with 10% FBS | Methods for Passages | 0.02% Trypsin + 0.01% EDTA |
| Cell Number on Passage | 1:3 - 1:5 /week | Race | Japanese |
| CO2 Conc. | Tissue Sampling | brain | |
| Tissue Type | glioblastoma |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:3188836 | Correlation of DNA ploidy and morphological features of human glioma cell cultures with the establishment of cell lines. Onda K,Tanaka R,Washiyama K,Takeda N,Kumanishi T Acta Neuropathol. 1988;76(5):433-40 |
| Pubmed id:none | GFAP-positive human glioma cell lines: no.11, no.10. Onda K., Washiyama K., Tanaka R., Nishiyama A., Usui H., Kumanishi T. Human Cell 3:251-252 (1990) |
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LOT Information
| Cell No. | JCRB1605 | Cell Name | Onda 11 |
|---|---|---|---|
| LOT No. | 09092014 | Lot Specification | distribution |
| Medium | DMEM with 10% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 0.48-1.1x10^4cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.02% trypsin and 0.01% EDTA.Split ratio=1/3-1/5 |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.70x10^6 |
| Viability at cell freezing (%) | 94.0 | Antibiotics Used | free |
| Passage Number | p52 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,11 D13S317:8,9 D7S820:11,12,13 D16S539:9,11 VWA:14,15,17 TH01:6,8 AM:X TPOX:8,11 CSF1PO:9,10,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information |
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