JCRB1625 HuSI-L23-14-5

Cell information

Cell type:genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1625 Cell Name   HuSI-L23-14-5
Profile   Human pre-immortalized cell line derived from MRC-5 cells. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   14-week-fetus
Identity   available Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   SV40 T-antigen transformed MRC-5
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   transformed Established by   Imai, S.
Registered by   Umezawa, A. Regulation for Distribution   
Comment    Year   2013
Medium   MEM medium with 10% FBS Methods for Passages   mechanical passage or 0.25% Trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   fetus lung
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_EG62
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LOT Information

Cell No.   JCRB1625 Cell Name   HuSI-L23-14-5
LOT No.   04202015 Lot Specification   distribution
Medium   MEM with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.5-3.30x10^4cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.40x10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:11
D7S820:10,11
D16S539:9,11
VWA:15
TH01:8
AM:X,Y
TPOX:8
CSF1PO:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
RFLP   NT Viability immediately after thawing (%)   
Additional information   
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