JCRB1640 HSC-1
Cell information
Important Notice(s)Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1640 | Cell Name | HSC-1 |
|---|---|---|---|
| Profile | Peripheral blood T lymphocyte cell line of cynomolgus monkey (immortalized by Herpesvirus saimiri). | Other Name | |
| Animal | Strain | ||
| Genus | Macaca | Species | fascicularis |
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | |
| Classify | transformed | Established by | Akari, H. |
| Registered by | Akari,H. | Regulation for Distribution | |
| Comment | Year | 2015 | |
| Medium | RPMI1640 with 10% fetal bovine serum, IL-2 (Interleukin-2, human). | Methods for Passages | Suspension culture (dilute 1/5) in 10mL of T25 flask vertical culture. This cell line should be cultured 3 - 10 x 10^5 cells/mL to keep good condition. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | peripheral blood lymphocytes |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:8610465 | In vitro immortalization of Old World monkey T lymphocytes with Herpesvirus saimiri: its susceptibility to infection with simian immunodeficiency viruses. Akari H,Mori K,Terao K,Otani I,Fukasawa M,Mukai R,Yoshikawa Y Virology. 1996 Apr 15;218(2):382-8 |
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LOT Information
| Cell No. | JCRB1640 | Cell Name | HSC-1 |
|---|---|---|---|
| LOT No. | 09182015 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) and IL-2(25-100 U/ml)(Roche Interleukin-2,human) | Temperature | 37 C |
| Cell Density at Seeding | 0.14-1.1x10^6 cells/ml | Methods for Passages | Dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 5.15x10^6 |
| Viability at cell freezing (%) | 97.00 | Antibiotics Used | free |
| Passage Number | p6* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | Cell Banker BLC-1(Nihon Zenyaku Industries) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information |
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