JCRB3028 WERI
Cell information
Cell type：human mutant cell repository from Kyoto University (View Pricing Information)
| JCRB No. | JCRB3028 | Cell Name | WERI |
|---|---|---|---|
| Profile | Hereditary disease with high cancer risk Retinoblastoma (autosomal dominant), Mycoplasma was eliminated with MC-210. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | Age | ||
| Identity | available | Tissue for Primary Cancer | tumor |
| Case history | Retinoblastoma | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | Crisis PDL | ||
| Morphology | Character | tumor cell line | |
| Classify | tumor | Established by | Dr. Inomata M. (National Cancer Center) |
| Registered by | Radiation Biology Center, Kyoto University | Regulation for Distribution | |
| Comment | Year | ||
| Medium | RPMI1640 containing 20% heat-inactivated fetal calf serum and 20uM β-mercaptoethanol | Methods for Passages | |
| Cell Number on Passage | Race | ||
| CO2 Conc. | Tissue Sampling | tumor | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
|||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Movies |
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LOT Information
| Cell No. | JCRB3028 | Cell Name | WERI |
|---|---|---|---|
| LOT No. | 09182015 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO) with 20uM β-Mercaptoetanol and 20% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 0.67-2.9x10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.62x10^6 |
| Viability at cell freezing (%) | 96.80 | Antibiotics Used | free |
| Passage Number | p10* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:14 D7S820:10,12,13 D16S539:13 VWA:17,18 TH01:9.3 AM:X TPOX:8,11 CSF1PO:11,12 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5 % |
| Viability immediately after thawing (%) | Additional information | Mycoplasma was eliminated with MC-210. |