JCRB1644 HSR-1

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1644 Cell Name   HSR-1
Profile   Peripheral blood lymphocyte cell line of rhesus monkey (immortalized by Herpesvirus saimiri). Other Name   
Animal   monkey Strain   
Genus   Macaca Species   mulatta
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   transformed Established by   Akari, H.
Registered by   Akari, H. Regulation for Distribution   
Comment    Year   2015
Medium   RPMI1640 with 10% fetal bovine serum, IL-2 (Interleukin-2, human). Methods for Passages   Suspension culture (dilute 1/5) in 10mL of T25 flask vertical culture. This cell line should be cultured 3 - 10 x 10^5 cells/mL to keep good condition.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   peripheral blood lymphocytes
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_9Q49
Reference
Pubmed id:8610465In vitro immortalization of Old World monkey T lymphocytes with Herpesvirus saimiri: its susceptibility to infection with simian immunodeficiency viruses.
Akari H,Mori K,Terao K,Otani I,Fukasawa M,Mukai R,Yoshikawa Y
Virology. 1996 Apr 15;218(2):382-8

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LOT Information

Cell No.   JCRB1644 Cell Name   HSR-1
LOT No.   10142015 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) and IL-2(50 U/ml)(Roche Interleukin-2,human) Temperature   37 C
Cell Density at Seeding   1.06-7.2x10^5cells/ml Methods for Passages   Dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   9.58x10^6
Viability at cell freezing (%)   92.4 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   Cell Banker BLC-1(Nihon Zenyaku Industries) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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