JCRB1648 ICH-ERMS-1

Cell information

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JCRB1648: ICH-ERMS-1 - Condition for Distribution [分譲の条件]

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1648 Cell Name   ICH-ERMS-1
Profile   Human cell derived from anaplastic embryonal rhabdomyosarcoma. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   5-year-old
Identity   available Tissue for Primary Cancer   sarcoma
Case history    Metastasis   
Tissue Metastasized    Genetics   TP53 c.743G>T
Life Span   infinite Crisis PDL   
Morphology   spindle or polygonal Character   
Classify   tumor Established by   Kato, K.
Registered by   Kato, K. Regulation for Distribution   Cite the litarature specified by the developer at the publication of papers using this cell line.
Comment    Year   2015
Medium   RPMI1640 medium with 10% FBS Methods for Passages   Trypsin/EDTA treatment
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   pelvic tumor
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_EG67
Reference
Pubmed id:33021000Distinct clonal evolution in a case with anaplastic embryonal rhabdomyosarcoma.
Kato K,Yoshimi A,Noda A,Otani H,Hojo H,Tanaka M,Tanaka Y,Ito Y,Nishimura R,Takita J,Yanai T,Koike K,Tsuchida M
Pediatr Int. 2021 Jul;63(7):782-789

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LOT Information

Cell No.   JCRB1648 Cell Name   ICH-ERMS-1
LOT No.   11242015 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.3-2.7x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.03x10^6
Viability at cell freezing (%)   97.7 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:12
D7S820:8,11
D16S539:12,13
VWA:14,18
TH01:9
AM:X
TPOX:8
CSF1PO:9
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5 %
Viability immediately after thawing (%)    Additional information   
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