JCRB0714.2 AH-7974.P3

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB0714.2 Cell Name   AH-7974.P3
Profile   Serum free rat ascited hepatoma cell line. Cells have been maintained by passage for long time. Extremely high passage number. (Date of deposit: 2015) Mycoplasma was eliminated with MC-210. Other Name   JTC-16.P3
Animal   rat Strain   Donryu
Genus   Rattus Species   norvegicus
Sex   M Age   
Identity    Tissue for Primary Cancer   liver
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span    Crisis PDL   
Morphology    Character   Ascites hepatoma, serum free cell culture.
Classify   variant Established by   Katsuta,H. & Takaoka,T.
Registered by   Takaoka,T. Regulation for Distribution   
Comment   This cells were definitly adapted to the serum free condition for long time. Number of passages are extremely high. Year   2015
Medium   DM-201 medium. Serum free. Methods for Passages   Cells are harvested with scraper
Cell Number on Passage   10^6 cells/ml Race   
CO2 Conc.   5 % Tissue Sampling   ascites
Tissue Type   hepatoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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LOT Information

Cell No.   JCRB0714.2 Cell Name   AH-7974.P3
LOT No.   02152016 Lot Specification   distribution
Medium   DM201 medium(Research Institute for the Functional peptides,lot No.15D2J),serum free condition. Temperature   37 C
Cell Density at Seeding   1.68-1.83x10^4 cells/sq.cm Methods for Passages   Cells are harvested with scraper.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.41x10^6
Viability at cell freezing (%)   88 Antibiotics Used   free
Passage Number   p16* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   FM-1and DM201 medium(1 to 1 mix) CO2 Conc.   5%
Viability immediately after thawing (%)   88 Additional information   Mycoplasma has eliminated with MC-210(Final concentratre 0.5 ug/ml).
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