JCRB1670 HT-1080/CMV-Luc

Cell information

Important Notice(s)

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1670 Cell Name   HT-1080/CMV-Luc
Profile   Luciferase stably expressing cell line (HT-1080; Human Sarcoma Cell Line with activated N-ras oncogene). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   35-year-old
Identity   available Tissue for Primary Cancer   sarcoma
Case history    Metastasis   
Tissue Metastasized    Genetics   The cells contain activated N-ras oncogene. pLVSIN-luc transfected HT-1080 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   puromycin-resistant
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2016
Medium   Eagle's Minimal Essential medium with non -essential amino acids and 10% fetal calf serum. Methods for Passages   Treat cells with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   dilute 1/4 - 1/8 Race   Caucasian
CO2 Conc.   5% Tissue Sampling   
Tissue Type   fibrosarcoma

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG56
Movies
JCRB1670_04012016.flv



LOT Information

Cell No.   JCRB1670 Cell Name   HT-1080/CMV-Luc
LOT No.   04012016 Lot Specification   distribution
Medium   MEM(GIBCO) with non-essential amino acids and 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.369-1.00x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.08x10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
D13S317:12,14
D7S820:9,10
D16S539:9,12
VWA:14,19
TH01:6
AM:X,Y
TPOX:8
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   98
Additional information   
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