JCRB1675 B16-F10/CMV-Luc#2

Cell information

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発光細胞 (ルシフェラーゼ発現細胞)について / Ordering Luc cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1675 Cell Name   B16-F10/CMV-Luc#2
Profile   Luciferase stably expressing cell line (B16-F10; Mouse Melanoma Cell Line). Other Name   
Animal   mouse Strain   C57BL/6J
Genus   Mus Species   musclus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   pLVSIN-luc transfected B16-F10 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   spindle-shape Character   
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2016
Medium   DMEM medium with 10% fetal bovine serum. Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio = 1/4 - 1/10 Race   
CO2 Conc.   5% Tissue Sampling   skin
Virus Summary    Virus Tested   
Tissue Type   
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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG40
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LOT Information

Cell No.   JCRB1675 Cell Name   B16-F10/CMV-Luc#2
LOT No.   05202016 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.5-5.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.61x10^6
Viability at cell freezing (%)   99.34 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   
Additional information   Ampoules were printed B16-F10/CMV-Luc.2
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Cell No.   JCRB1675 Cell Name   B16-F10/CMV-Luc#2
LOT No.   11152019 Lot Specification   distribution
Medium   DMEM(GIBCO,Cat.No.11885-084) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.5-4.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.18x10^6
Viability at cell freezing (%)   98.67 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   Ampoules were printed B16-F10/CMV-Luc.2
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