JCRB1679 HeLa/CMV-Luc

Cell information

Important Notice(s)

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines

発光細胞へ導入された遺伝子等の情報はこちら

Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1679 Cell Name   HeLa/CMV-Luc
Profile   Luciferase stably expressing cell line (HeLa; Human Cervical Carcinoma Cell Line). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   31-year-old
Identity   not done Tissue for Primary Cancer   uterine cervix
Case history   carcinoma of cervix Metastasis   
Tissue Metastasized    Genetics   pLVSIN-luc transfected HeLa cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   first establish cell line in vitro from human. puromycin-resistant.
Classify   tumor Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2016
Medium   Eagle's minimum essential medium (Earle's salts, containing L-glutamine) with non essential amino acids and 10 % fetal bovine serum. Methods for Passages   Cells harvested after treated with 0.02% EDTA and 0.25% trypsin.
Cell Number on Passage   split ratio=1/2 - 1/10 Race   Black
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG57
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LOT Information

Cell No.   JCRB1679 Cell Name   HeLa/CMV-Luc
LOT No.   06062016 Lot Specification   distribution
Medium   MEM(GIBCO) with non-essential amino acids(GIBCO) and 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   3.37-3.39x10^3 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.59x10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   p3* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:12,13.3
D7S820:8,12
D16S539:9,10
VWA:16,18
TH01:7
AM:X
TPOX:8,12
CSF1PO:9,10
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94
Additional information   
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