JCRB1681 MIA PaCa-2/CMV-Luc

Cell information

Important Notice(s)

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines


Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1681 Cell Name   MIA PaCa-2/CMV-Luc
Profile   Luciferase stably expressing cell line (MIA PaCa-2; Human Pancreatic Cancer Cell Line). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   65-year-old
Identity   not done Tissue for Primary Cancer   pancreas
Case history   pancreatic cancer Metastasis   
Tissue Metastasized    Genetics   pLVSIN-luc transfected MIA PaCa-2 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   2016
Medium   Eagle's minimal essential medium with non-essential a.a. and 10% fetal calf serum(heat inactivated) Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin.
Cell Number on Passage   split ratio=1/3 - 1/4 Race   
CO2 Conc.   5% Tissue Sampling   pancreas
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG54

LOT Information

Cell No.   JCRB1681 Cell Name   MIA PaCa-2/CMV-Luc
LOT No.   06102016 Lot Specification   distribution
Medium   Eagle's minimal essential medium with non-essential amino acids(0.1mM) and 10% heat-inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.8-2.0x10^5 cells/sq.cm Methods for Passages   Cells are treated with 0.02 % EDTA and 0.25 % trypsin. subculture once a week
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.6x10^6
Viability at cell freezing (%)   96.3 Antibiotics Used   free
Passage Number   p2* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5 %
RFLP    Viability immediately after thawing (%)   NT
Additional information