JCRB0144.2D LYM-1.P3D

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB0144.2D Cell Name   LYM-1.P3D
Profile   Serum free FAR-2.F60 rat cell culture from lymph node near colon cancer. Other Name   
Animal   rat Strain   JAR-2
Genus   Rattus Species   norvegicus
Sex    Age   
Identity    Tissue for Primary Cancer   
Case history   tissue obtained from lymph node near colon cancer of the JAR-2 rat given MNU(0.4mg/ml). The MNU was applied to a bowel directly of two-week-old rat and tumors were found three mo. later. The lymph node was cultured by roller tubes with DM170 and 2% FBS. Metastasis   
Tissue Metastasized    Genetics   Subclone of the LYM-1.P3. Morphologys and karyotypes were different from the LYM-1.P3B. Karyotype is lower diploid.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Culture fruid of the LYM-1 strongly inhibited a growth of AH-7974 and other several cell lines. The inhibition was strongly suppressed by heating.
Classify   serum free Established by   Kurayama,H. and Takaoka,T.
Registered by   Takaoka,T. Regulation for Distribution   free
Comment   from LYM-1 Year   2015
Medium   DM201 medium with serum free condition. Methods for Passages   Cells harvested by gentle scraping.
Cell Number on Passage   1.3 x 10^4 cells/sq.cm. Race   
CO2 Conc.    Tissue Sampling   lymph node near colon cancer
Virus Summary    Virus Tested   
Tissue Type   
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_U215

LOT Information

Cell No.   JCRB0144.2D Cell Name   LYM-1.P3D
LOT No.   04132016 Lot Specification   distribution
Medium   DM201 medium(Research Institute for the Functional peptides).Lipid and protein free condition. Temperature   37 C
Cell Density at Seeding   2.5-3.27x10^4 cells/sq.cm Methods for Passages   Cells were harvested with scraper.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.84x10^6
Viability at cell freezing (%)   95 Antibiotics Used   free
Passage Number   p9* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   FM-1 and DM201 medium(1/1) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   90
Additional information