JCRB1692 HuO9 CMV-Luc

Cell information

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発光細胞 (ルシフェラーゼ発現細胞)について / Ordering Luc cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1692 Cell Name   HuO9 CMV-Luc
Profile   Luciferase stably expressing cell line (HuO9;A cell line established from a human osteosarcoma transplanted into athymic nude mice after fifth passages. The cell line expressed 1,25(OH)2D3-responsive alikaline phosphatase and produced tumors in the dorsum of nude mice). Other Name   HuO9
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   13
Identity   available Tissue for Primary Cancer   sarcoma
Case history   osteosarcoma Metastasis   
Tissue Metastasized    Genetics   pLVSIN-luc transfected HuO9 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Secretes osteocalcin & responds to vitamin D3. Liver/bone/kidney-type alkaline phosphatase.
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells treated with 0.2 % trypsin and 0.02% EDTA.
Cell Number on Passage   1-4 x 10^5 cells/ml Race   
CO2 Conc.   5% Tissue Sampling   bone (right femur)
Virus Summary    Virus Tested   
Tissue Type   
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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG37
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LOT Information

Cell No.   JCRB1692 Cell Name   HuO9 CMV-Luc
LOT No.   07162016 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.995-2.20x10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.2%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.00x10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
D13S317:11
D7S820:10
D16S539:11
VWA:14
TH01:9
AM:X
TPOX:9
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94
Additional information   
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