JCRB1692 HuO9 CMV-Luc

Cell information

Important Notice(s)

発光細胞 (ルシフェラーゼ発現細胞)の分譲について / Ordering Luc cell lines


Information for introduced genes, etc in luciferase expressing cell lines

Cell type:luciferase-expressing cancer cells (View Pricing Information)

JCRB No.   JCRB1692 Cell Name   HuO9 CMV-Luc
Profile   Luciferase stably expressing cell line (HuO9;A cell line established from a human osteosarcoma transplanted into athymic nude mice after fifth passages. The cell line expressed 1,25(OH)2D3-responsive alikaline phosphatase and produced tumors in the dorsum of nude mice). Other Name   HuO9
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   13
Identity   available Tissue for Primary Cancer   sarcoma
Case history   osteosarcoma Metastasis   
Tissue Metastasized    Genetics   pLVSIN-luc transfected HuO9 cells. Luciferase stably expressing cell line.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   Secretes osteocalcin & responds to vitamin D3. Liver/bone/kidney-type alkaline phosphatase. Puromycin-resistant.
Classify   transformed Established by   Murakami, T.
Registered by   Murakami, T. Regulation for Distribution   To exchange and follow MTA between oversea end-user and cell-developer.
Comment    Year   
Medium   RPMI1640 medium with 10% fetal bovine serum. Methods for Passages   Cells treated with 0.2 % trypsin and 0.02% EDTA.
Cell Number on Passage   1-4 x 10^5 cells/ml Race   
CO2 Conc.   5% Tissue Sampling   bone (right femur)
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG37

LOT Information

Cell No.   JCRB1692 Cell Name   HuO9 CMV-Luc
LOT No.   07162016 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   0.995-2.20x10^4 cells/ml Methods for Passages   Cells were harvested after treatment with 0.2%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.00x10^6
Viability at cell freezing (%)   98 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   94
Additional information