JCRB1694 HSAt-005

Cell information

Important Notice(s)

JCRB1694 HSAt-005の培養について（培養難易度高）

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1694 Cell Name   HSAt-005
Profile   Peripheral blood lymphocyte cell line of Night Monkey (immortalized by Herpesvirus saimiri). Other Name   Owl Monkey (Night Monkey)
Animal   monkey Strain   
Genus   Aotus Species   trivirgatus
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   transformed Established by   Akari, H.
Registered by   Akari,H. Regulation for Distribution   Restricted by Washington Treaty
Comment    Year   2015
Medium   RPMI1640 with 10% fetal bovine serum, IL-2 (Interleukin-2, human). Methods for Passages   Suspension culture (dilute 1/5) in 10mL of T25 flask vertical culture. This cell line should be cultured 3 - 10 x 10^5 cells/mL to keep good condition.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   peripheral blood lymphocytes
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_JG01
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LOT Information

Cell No.   JCRB1694 Cell Name   HSAt-005
LOT No.   09212021 Lot Specification   distribution
Medium   RPMI1640(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum(SIGMA12J396) and IL-2(100 u/ml)(PEPRO: :IL-2,human reconbinant) Temperature   37 C
Cell Density at Seeding   0.5-0.9x10^5 cells/ml Methods for Passages   Dilution (split ratio=1/2)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   97.60 Antibiotics Used   free
Passage Number   p21* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   87.4 Additional information   Keep cell density under 2x10^5 cells/ml,subuculture or add IL-2 every 3-4days. Type of ampoules is plastic.
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