JCRB1702 CHO-WBL(IVGT)
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1702 | Cell Name | CHO-WBL(IVGT) |
|---|---|---|---|
| Profile | The Chinese hamster ovary CHO cells were originally established as spontaneously transformed cells from an ovarian biopsy by Tijo and Puck. | Other Name | |
| Animal | hamster, Chinese | Strain | |
| Genus | Species | ||
| Sex | F | Age | |
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | ||
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | polygonal-fibroblast | Character | |
| Classify | transformed | Established by | Merck Sharp and Dohme Corp |
| Registered by | ECACC | Regulation for Distribution | |
| Comment | Year | ||
| Medium | McCoy′s 5a + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). | Methods for Passages | Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Pipette to break up clumps. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | overy |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:13598821 | Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects. PUCK TT,CIECIURA SJ,ROBINSON A J Exp Med. 1958 Dec 1;108(6):945-56 |
| Pubmed id:13563760 | Genetics of somatic mammalian cells. II. Chromosomal constitution of cells in tissue culture. TJIO JH,PUCK TT J Exp Med. 1958 Aug 1;108(2):259-68 |
| Research results by users. Click！ | |
|---|---|
| Pubmed id:10320750 |
Characterization of p53 in Chinese hamster cell lines CHO-K1, CHO-WBL, and CHL: implications for genotoxicity testing. Hu T,Miller CM,Ridder GM,Aardema MJ Mutat Res. 1999 May 3;426(1):51-62 |
| Pubmed id:2253606 |
Chromosome aberration and sister chromatid exchange tests in Chinese hamster ovary cells in vitro. V: Results with 46 chemicals. Loveday KS,Anderson BE,Resnick MA,Zeiger E Environ Mol Mutagen. 1990;16(4):272-303 |
| Pubmed id:2792092 |
Chromosomal aberrations and sister chromatid exchange tests in Chinese hamster ovary cells in vitro. IV. Results with 15 chemicals. Ivett JL,Brown BM,Rodgers C,Anderson BE,Resnick MA,Zeiger E Environ Mol Mutagen. 1989;14(3):165-87 |
| Pubmed id:3319609 |
Chromosome aberrations and sister chromatid exchanges in Chinese hamster ovary cells: evaluations of 108 chemicals. Galloway SM,Armstrong MJ,Reuben C,Colman S,Brown B,Cannon C,Bloom AD,Nakamura F,Ahmed M,Duk S Environ Mol Mutagen. 1987;10 Suppl 10():1-175 |
| Pubmed id:3967632 |
Development of a standard protocol for in vitro cytogenetic testing with Chinese hamster ovary cells: comparison of results for 22 compounds in two laboratories. Galloway SM,Bloom AD,Resnick M,Margolin BH,Nakamura F,Archer P,Zeiger E Environ Mutagen. 1985;7(1):1-51 |
LOT Information
| Cell No. | JCRB1702 | Cell Name | CHO-WBL(IVGT) |
|---|---|---|---|
| LOT No. | 15L029 | Lot Specification | distribution |
| Medium | McCoy′s 5a + 2mM Glutamine + 10% Fetal Bovine Serum (FBS). | Temperature | 37 C |
| Cell Density at Seeding | Methods for Passages | Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.05% trypsin or trypsin/EDTA; 5% CO2; 37°C. Pipette to break up clumps. | |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | |
| Viability at cell freezing (%) | Antibiotics Used | ||
| Passage Number | PDL | NT | |
| Sterility: MYCOPLASMA | Sterility: BACTERIA | ||
| Sterility: FUNGI | Isozyme Analysis | NT | |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | Exoteric Gene | NT | |
| Medium for Freezing | CO2 Conc. | 5% | |
| Viability immediately after thawing (%) | NT | Additional information |