JCRB1713 KM8
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1713 | Cell Name | KM8 |
|---|---|---|---|
| Profile | Human fibroblast cell Line with mutation of LDL receptor | Other Name | |
| Animal | human | Strain | |
| Genus | Species | ||
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | N | |
| Tissue Metastasized | Genetics | LDL receptor mutation (exon 9, c.1297 G > C, Homozygote) | |
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | LDL receptor mutation D412H (born from consanguineous marriage), Receptor negative type, Rapid degradation of synthesized receptor |
| Classify | primary | Established by | Yasuko Miyake |
| Registered by | Ken-ichi Hirano | Regulation for Distribution | |
| Comment | Year | 2016 | |
| Medium | DMEM with 10% FBS | Methods for Passages | Trypsin-EDTA. split ratio = 1:1. Once/3-4 days. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | skin |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1446662 | A point mutation of low-density-lipoprotein receptor causing rapid degradation of the receptor. Miyake Y,Tajima S,Funahashi T,Yamamura T,Yamamoto A Eur J Biochem. 1992 Nov 15;210(1):1-7 |
| Pubmed id:3391611 | Mutations of the low density lipoprotein receptor in Japanese kindreds with familial hypercholesterolemia. Funahashi T,Miyake Y,Yamamoto A,Matsuzawa Y,Kishino B Hum Genet. 1988 Jun;79(2):103-8 |
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LOT Information
| Cell No. | JCRB1713 | Cell Name | KM8 |
|---|---|---|---|
| LOT No. | 11082016 | Lot Specification | distribution |
| Medium | DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 2.08-3.80x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.44x10^6 |
| Viability at cell freezing (%) | 99 | Antibiotics Used | free |
| Passage Number | p17 | PDL | 20 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:11,12 D13S317:10,12 D7S820:8,11 D16S539:11,14 VWA:17,18 TH01:9 AM:X,Y TPOX:8,11 CSF1PO:10,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information |
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