JCRB1713 KM8

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1713 Cell Name   KM8
Profile   Human fibroblast cell Line with mutation of LDL receptor Other Name   
Animal   human Strain   
Genus    Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   N
Tissue Metastasized    Genetics   LDL receptor mutation (exon 9, c.1297 G > C, Homozygote)
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   LDL receptor mutation D412H (born from consanguineous marriage), Receptor negative type, Rapid degradation of synthesized receptor
Classify   primary Established by   Yasuko Miyake
Registered by   Ken-ichi Hirano Regulation for Distribution   
Comment    Year   2016
Medium   DMEM with 10% FBS Methods for Passages   Trypsin-EDTA. split ratio = 1:1. Once/3-4 days.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   skin
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_RY75
Reference
Pubmed id:1446662 A point mutation of low-density-lipoprotein receptor causing rapid degradation of the receptor.
Miyake Y,Tajima S,Funahashi T,Yamamura T,Yamamoto A
Eur J Biochem. 1992 Nov 15;210(1):1-7
Pubmed id:3391611 Mutations of the low density lipoprotein receptor in Japanese kindreds with familial hypercholesterolemia.
Funahashi T,Miyake Y,Yamamoto A,Matsuzawa Y,Kishino B
Hum Genet. 1988 Jun;79(2):103-8

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LOT Information

Cell No.   JCRB1713 Cell Name   KM8
LOT No.   11082016 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   2.08-3.80x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.44x10^6
Viability at cell freezing (%)   99 Antibiotics Used   free
Passage Number   p17 PDL   20
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
D13S317:10,12
D7S820:8,11
D16S539:11,14
VWA:17,18
TH01:9
AM:X,Y
TPOX:8,11
CSF1PO:10,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
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