--- Lewis lung carcinoma (3LL): Culture medium ---
The culture medium used for propagation of Lewis lung carcinoma and related cell lines (listed below) is RPMI 1640 medium with 10% fetal bovine serum.
JCRB1348 3LL
JCRB1349 Ex-3LL
JCRB1351 Int-3LL
Originally, these cell lines were reported to be cultivated using Ham's F10 : Leivovitz L15 = 3:7 medium with 10% fetal bovine serum. When the cell lines were deposited at cell bank, however, these cell lines were cultivated in RPMI 1640 medium with 10% fetal bovine serum.
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[発光細胞 (ルシフェラーゼ発現細胞)について]
本細胞株は営利団体への分譲に際して樹立者による事前の承諾が必要です。
分譲依頼書を受領後、バンクが樹立者からの承諾を得ます。
分譲依頼書の研究目的は、お知らせ頂いて差支えない範囲で出来るだけ詳細にご記載下さい。
発光細胞については、論文発表に際して、細胞樹立者の村上先生から細胞の提供を受けたことに関する謝辞を記して頂くようお願いしております。
細胞樹立者
村上 孝 (Takashi Murakami M.D., Ph.D.)
〒350-0495
埼玉県入間郡毛呂山町毛呂本郷38
埼玉医科大学医学部 微生物学
[When ordering Luciferase-expressing cancer cells]
The distribution of Luc cell lines requires an additional MTA between overseas users and the developer/depositor.
MTA form will be sent to applicants from Cell Bank upon request of distribution.
Please describe the research purposes in detail when ordering.
For details, please contact JCRB cell bank.
Developer/Depositor: Takashi Murakami, M.D., Ph.D.
Saitama Medical University, Faculty of Medicine
38 Moro-Hongo, Moroyama, Iruma, Saitama 350-0495, Japan
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Information for luciferase gene-introduced cell lines
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