JCRB1721 NCMDF

Cell information

Important Notice(s)

JCRB1721 NCMDF細胞の分譲について

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1721 Cell Name   NCMDF
Profile   Normal Cynomolgus Monkey Dermal Fibroblasts Other Name   
Animal   monkey, Cynomologus Strain   
Genus   Macaca Species   fascicularis
Sex   M Age   21-month-old
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   Normal Fibroblast
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   primary Established by   Matsuo, J.
Registered by   Matsuo, J. Regulation for Distribution   Restricted by Washington Treaty. End-user information is notified to the depositor.
Comment   The monkey that this cell line originated from was anti-herpes simian B virus (Macacine herpesvirus 1) antibody negative (tested by the establisher's organization). Year   2016
Medium   DMEM with 20% heat-inactivated fetal bovine serum Methods for Passages   0.25% Trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   skin (abdomen)
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_LC41
Images
Movies



LOT Information

Cell No.   JCRB1721 Cell Name   NCMDF
LOT No.   02142017 Lot Specification   distribution
Medium   DMEM(GIBCO) with 20% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   1.00-3.20x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.31x10^6
Viability at cell freezing (%)   99.70 Antibiotics Used   free
Passage Number   p5* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
Images
Cell No.   JCRB1721 Cell Name   NCMDF
LOT No.   01152019 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 20% fetal bovine serum (FBS; Sigma Cat. # 172012) Temperature   37 C
Cell Density at Seeding   3.3 - 4.5 x 10^4 Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 0.02% EDTA.
Doubling Time   approx. 16 hrs. Cell Number in Vial (cells/1ml)   2.0 x 10^6
Viability at cell freezing (%)   97.8 Antibiotics Used   free
Passage Number   Unknown (6 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - DMEM CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   89.6
Additional information   
CLOSE