JCRB0152.4 M.P3 Cell information
Cell type：general cells
(View Pricing Information )
JCRB No.
JCRB0152.4
Cell Name
M.P3
Profile
M cells derived from RLC-3 (JAR rat) was habituated to serum free culture medium.
Other Name
Animal
rat
Strain
JAR-1
Genus
Rattus
Species
norvegicus
Sex
Age
10-day-old
Identity
not done
Tissue for Primary Cancer
Case history
normal
Metastasis
Tissue Metastasized
Genetics
4-dimethylaminoazobenzene(DAB) transformed and remarkable changes were found in the biochemical properties.
Life Span
infinite
Crisis PDL
Morphology
epithelial-like
Character
Morphological changes observed cell line by DAB treatment. Historical cell line produced by Nagisa culture and DAB treatment.
Classify
transformed
Established by
Katsuta,H. and Takaoka,T.
Registered by
Takaoka,T.
Regulation for Distribution
free
Comment
Cells grow in serum free medium established by Takaoka,T. and Katsuta,H.
Year
2015
Medium
DM201 medium. (This culture requires serum free medium definitely).
Methods for Passages
Cells harvested by scraper and hard to individualize cells. Additional information is under preparation.
Cell Number on Passage
Split ratio = 1/2.
Race
CO2 Conc.
Tissue Sampling
liver
Tissue Type
tumor cells by DAB with Nagisa culture
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Movies
LOT Information
Cell No.
JCRB0152.4
Cell Name
M.P3
LOT No.
03012017
Lot Specification
distribution
Medium
DM201 medium(Research Institute for the Functional peptides),Lipid and protein free condition.
Temperature
37 C
Cell Density at Seeding
NT
Methods for Passages
Harvest cells with scraper.
1/1-1/5 split
Doubling Time
NT
Cell Number in Vial (cells/1ml)
NT
Viability at cell freezing (%)
NT
Antibiotics Used
free
Passage Number
p27*
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
FM-1 (Kyokuto Pharmaceutical Industrial Co.,Ltd.:
#551-27100-3)
and DM201 medium (1 to 1 mix)
CO2 Conc.
5%
Viability immediately after thawing (%)
NT
Additional information
Mycoplasma has eliminated with MC-210(Final concentratre 0.5 ug/ml).
