JCRB1726 AK28
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1726 | Cell Name | AK28 |
|---|---|---|---|
| Profile | Human fibroblast cell Line with mutation of LDL receptor. | Other Name | |
| Animal | human | Strain | |
| Genus | Species | ||
| Sex | Age | ||
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | N | |
| Tissue Metastasized | Genetics | LDL receptor mutation (intron 12, c.1845+2 T>C, Heterozygote (born from consanguineous marriage)) | |
| Life Span | finite | Crisis PDL | |
| Morphology | fibroblast-like | Character | LDL receptor mutation, intro 12, 5’splice donor mutation, Heterozygote (born from consanguineous marriage), Receptor negative type. |
| Classify | primary | Established by | Yasuko Miyake |
| Registered by | Ken-ichi Hirano | Regulation for Distribution | |
| Comment | Year | 2016 | |
| Medium | DMEM with 10% FBS | Methods for Passages | Trypsin-EDTA. split ratio = 1:1. Once/3-4 days. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | tested | ||||||
| HCV | - | HTLV-1 | - | ||||||
| HIV-1 | - | HTLV-2 | - | ||||||
| HIV-2 | - | HAV | - | ||||||
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-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:7583548 | Common mutations in the low-density-lipoprotein-receptor gene causing familial hypercholesterolemia in the Japanese population. Maruyama T,Miyake Y,Tajima S,Harada-Shiba M,Yamamura T,Tsushima M,Kishino B,Horiguchi Y,Funahashi T,Matsuzawa Y Arterioscler Thromb Vasc Biol. 1995 Oct;15(10):1713-8 |
| Pubmed id:7588706 | A single point mutation in the splice donor site of the low-density-lipoprotein-receptor gene produces intron read-through, exon-skipped and cryptic-site-utilized transcripts. Maruyama T,Miyake Y,Tajima S,Funahashi T,Matsuzawa Y,Yamamoto A Eur J Biochem. 1995 Sep 15;232(3):700-5 |
| Pubmed id:3391611 | Mutations of the low density lipoprotein receptor in Japanese kindreds with familial hypercholesterolemia. Funahashi T,Miyake Y,Yamamoto A,Matsuzawa Y,Kishino B Hum Genet. 1988 Jun;79(2):103-8 |
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LOT Information
| Cell No. | JCRB1726 | Cell Name | AK28 |
|---|---|---|---|
| LOT No. | 04042017 | Lot Specification | distribution |
| Medium | DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) | Temperature | 37 C |
| Cell Density at Seeding | 2.0-4.0x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.49 |
| Viability at cell freezing (%) | 99.47 | Antibiotics Used | free |
| Passage Number | p10 | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,13 D13S317:11,13 D7S820:10 D16S539:12,13 VWA:17,18 TH01:6,9 AM:X TPOX:8,12 CSF1PO:12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | NT | Additional information |
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