JCRB1726 AK28

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1726 Cell Name   AK28
Profile   Human fibroblast cell Line with mutation of LDL receptor. Other Name   
Animal   human Strain   
Genus    Species   
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   N
Tissue Metastasized    Genetics   LDL receptor mutation (intron 12, c.1845+2 T>C, Heterozygote (born from consanguineous marriage))
Life Span   finite Crisis PDL   
Morphology   fibroblast-like Character   LDL receptor mutation, intro 12, 5’splice donor mutation, Heterozygote (born from consanguineous marriage), Receptor negative type.
Classify   primary Established by   Yasuko Miyake
Registered by   Ken-ichi Hirano Regulation for Distribution   
Comment    Year   2016
Medium   DMEM with 10% FBS Methods for Passages   Trypsin-EDTA. split ratio = 1:1. Once/3-4 days.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus tested
HCV - HTLV-1 -
HIV-1 - HTLV-2 -
HIV-2 - HAV -
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_LC42
Pubmed id:7583548Common mutations in the low-density-lipoprotein-receptor gene causing familial hypercholesterolemia in the Japanese population.
Maruyama T,Miyake Y,Tajima S,Harada-Shiba M,Yamamura T,Tsushima M,Kishino B,Horiguchi Y,Funahashi T,Matsuzawa Y
Arterioscler Thromb Vasc Biol. 1995 Oct;15(10):1713-8
Pubmed id:7588706A single point mutation in the splice donor site of the low-density-lipoprotein-receptor gene produces intron read-through, exon-skipped and cryptic-site-utilized transcripts.
Maruyama T,Miyake Y,Tajima S,Funahashi T,Matsuzawa Y,Yamamoto A
Eur J Biochem. 1995 Sep 15;232(3):700-5
Pubmed id:3391611Mutations of the low density lipoprotein receptor in Japanese kindreds with familial hypercholesterolemia.
Funahashi T,Miyake Y,Yamamoto A,Matsuzawa Y,Kishino B
Hum Genet. 1988 Jun;79(2):103-8


LOT Information

Cell No.   JCRB1726 Cell Name   AK28
LOT No.   04042017 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   2.0-4.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.49
Viability at cell freezing (%)   99.47 Antibiotics Used   free
Passage Number   p10 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information