JCRB1736 293_hSyn1

Cell information

Important Notice(s)

This cell line is available for only academic users.

本細胞は学術機関へのみの分譲となります

本細胞の分譲条件について

The condition of distribution of this cell line

Cell type:genetically-modified cells (View Pricing Information)

JCRB No.   JCRB1736 Cell Name   293_hSyn1
Profile   293 cell line into which human Syndecan-1 gene has been transfected. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex    Age   
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology    Character   G418 resistant (0.5mg/mL), p3 x FLAG CMV-10(Sigma)
Classify   transformed Established by   Mochitate K. & Nakamura N.
Registered by   Mochitate Katsumi Regulation for Distribution   Available for non-profit organizations only
Comment    Year   2017
Medium   DMEM with 5-10% FBS and G418. Methods for Passages   0.05% Trypsin & 0.53mM EDTA or Accutase(better)
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   kidney
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus not tested
CMV - parvoB19 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
EBV - HBV -
HHV6 - HTLV-1 -
HHV7 - HTLV-2 -
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_RY72
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LOT Information

Cell No.   JCRB1736 Cell Name   293_hSyn1
LOT No.   06262017 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(SIGMA12J396) and 0.5 mg/ml G-418 Temperature   37 C
Cell Density at Seeding   2.3-3.7x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25% trypsin and 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.44x10^6
Viability at cell freezing (%)   98.4 Antibiotics Used   free
Passage Number   p7 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:8
D13S317:12,14
D7S820:11
D16S539:9,13
VWA:16,19
TH01:7,9.3
AM:X
TPOX:11
CSF1PO:12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
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