JCRB1737 In-1024 Cell information
Cell type：general cells
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JCRB No.
JCRB1737
Cell Name
In-1024
Profile
Insulinoma cell line of Syrian hamster induced by BK virus. (Insulin secretion in vitro was not found by JCRB's ELISA test.)
Other Name
Animal
hamster, Syrian
Strain
Syrian
Genus
Mesocricetus
Species
auratus
Sex
Age
Identity
not done
Tissue for Primary Cancer
pancreas
Case history
Insulinoma induced by intracerebroventricular injection of BK virus.
Metastasis
Tissue Metastasized
Genetics
Life Span
infinite
Crisis PDL
Morphology
polygonal-epithelial
Character
Insulin secretion in vitro was not found by JCRB's ELISA test.
Classify
tumor
Established by
Uchida S.
Registered by
Kousuke Kataoka
Regulation for Distribution
use in Japan only
Comment
Year
2017
Medium
Dulbecco's modified Eagle's medium with 10% fetal calf serum
Methods for Passages
Cells were harvested after treatment with 0.05% trypsin and 0.002% EDTA inPBS(-).
Cell Number on Passage
split ratio = 1/20
Race
CO2 Conc.
5%
Tissue Sampling
pancreas
Tissue Type
Detection of virus genome fragment by Real-time PCR
Detected DNA Virus
not tested
Detected RNA Virus
not tested
Movies
LOT Information
Cell No.
JCRB1737
Cell Name
In-1024
LOT No.
07122017
Lot Specification
distribution
Medium
Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(SIGMA12J396)
Temperature
37 C
Cell Density at Seeding
1.00-2.00x10^4 cells/sq.cm
Methods for Passages
Cells were harvested after treatment with 0.05% trypsin and 0.002% EDTA
Doubling Time
NT
Cell Number in Vial (cells/1ml)
2.02x10^6
Viability at cell freezing (%)
97.31
Antibiotics Used
free
Passage Number
p4*
PDL
NT
Sterility: MYCOPLASMA
-
Sterility: BACTERIA
-
Sterility: FUNGI
-
Isozyme Analysis
NT
Chromosome Mode
NT
Chromosome Information
NT
Surface Antigen
NT
DNA Profile (STR)
Adhesion
Yes
Exoteric Gene
NT
Medium for Freezing
BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD)
CO2 Conc.
5%
Viability immediately after thawing (%)
NT
Additional information
