JCRB1772 KKU-452

Cell information

Important Notice(s)

KKU-023, KKU-452細胞の分譲条件について

On the distribution of KKU-023 and KKU-452 cell lines.

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1772 Cell Name   KKU-452
Profile   Human poorly differentiated cholangiocarcinoma cell line established from biliary tract. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   63-year-old
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   Y
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Veerapol Kukongviriyapan
Registered by   Poramate Klanrit Regulation for Distribution   
Comment    Year   
Medium   Ham’s F12 medium supplemented with 10% fetal bovine serum (FBS), Minimum Essential Medium (MEM) non-essential amino acid, and 12.5 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES). Methods for Passages   Trypsin-EDTA
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_VF99
Pubmed id:29110582Establishment of cholangiocarcinoma cell lines from patients in the endemic area of liver fluke infection in Thailand.
Saensa-Ard S,Leuangwattanawanit S,Senggunprai L,Namwat N,Kongpetch S,Chamgramol Y,Loilome W,Khansaard W,Jusakul A,Prawan A,Pairojkul C,Khantikeo N,Yongvanit P,Kukongviriyapan V
Tumour Biol. 2017 Nov;39(11):1010428317725925


LOT Information

Cell No.   JCRB1772 Cell Name   KKU-452
LOT No.   11052018 Lot Specification   distribution
Medium   Ham's F12 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396),NEAA(GIBCO#11140050) and 12.5 mM HEPES(WaKo#3345-06681) Temperature   37 C
Cell Density at Seeding   1.18-1.52x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.11x10^6
Viability at cell freezing (%)   96 Antibiotics Used   free
Passage Number   p75 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   80
Additional information