JCRB1775 KKK-D068

Cell information

Important Notice(s)

About the citation of KKK-series of cell lines.

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1775 Cell Name   KKK-D068
Profile    Human well differentiated tubular adenocarcinma cell line established from biliary tract. (with micropapillary foci) Other Name   KKK-D068H1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   61
Identity   not done Tissue for Primary Cancer   liver, gallbladder
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Vaeteewoottacharn, K.
Registered by   Okada, S. Regulation for Distribution   
Comment    Year   2014
Medium   DMEM with 10% fetal bovine serum. Methods for Passages   0.25% Trypsin - 0.02% EDTA
Cell Number on Passage   Split ratio 1:3 - 1:5 Race   
CO2 Conc.   5% Tissue Sampling   Intrahepatic bile duct
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_XD18
Pubmed id:31077409Dependency of Cholangiocarcinoma on Cyclin D-Dependent Kinase Activity.
Sittithumcharee G,Suppramote O,Vaeteewoottacharn K,Sirisuksakun C,Jamnongsong S,Laphanuwat P,Suntiparpluacha M,Matha A,Chusorn P,Buraphat P,Kakanaporn C,Charngkaew K,Silsirivanit A,Korphaisarn K,Limsrichamrern S,Tripatara P,Pairojkul C,Wongkham S,Sampattavanich S,Okada S,Jirawatnotai S
Hepatology. 2019 Nov;70(5):1614-1630
Pubmed id:31126020Establishment of Highly Transplantable Cholangiocarcinoma Cell Lines from a Patient-Derived Xenograft Mouse Model.
Vaeteewoottacharn K,Pairojkul C,Kariya R,Muisuk K,Imtawil K,Chamgramol Y,Bhudhisawasdi V,Khuntikeo N,Pugkhem A,Saeseow OT,Silsirivanit A,Wongkham C,Wongkham S,Okada S
Cells. 2019 May 23;8(5):


LOT Information

Cell No.   JCRB1775 Cell Name   KKK-D068
LOT No.   12072018 Lot Specification   distribution
Medium   Dulbecco's modified Eagle's medium with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   2-2.5x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.(Split 1/2)
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.55x10^6
Viability at cell freezing (%)   97.5 Antibiotics Used   free
Passage Number   p26 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   89.6 Additional information   Vials are labeled as KKK-D068 H1.