JCRB1777 KKK-D131

Cell information

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About the citation of KKK-series of cell lines.

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1777 Cell Name   KKK-D131
Profile   Human well differentiated tubular adenocarcinoma cell line established from biliary tract. Other Name   KKK-D131H1
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   66
Identity   not done Tissue for Primary Cancer   liver, gallbladder
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Vaeteewoottacharn, K.
Registered by   Okada, S. Regulation for Distribution   
Comment    Year   2014
Medium   DMEM with 10% fetal bovine serum Methods for Passages   0.25% Trypsin - 0.02% EDTA
Cell Number on Passage   split ratio 1:3 - 1:5 Race   
CO2 Conc.   5% Tissue Sampling   intrahepatic bile duct
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Reference
Pubmed id:31126020 Establishment of Highly Transplantable Cholangiocarcinoma Cell Lines from a Patient-Derived Xenograft Mouse Model.
Vaeteewoottacharn K,Pairojkul C,Kariya R,Muisuk K,Imtawil K,Chamgramol Y,Bhudhisawasdi V,Khuntikeo N,Pugkhem A,Saeseow OT,Silsirivanit A,Wongkham C,Wongkham S,Okada S
Cells. 2019 May 23;8(5):
Pubmed id:31077409 Dependency of Cholangiocarcinoma on Cyclin D-Dependent Kinase Activity.
Sittithumcharee G,Suppramote O,Vaeteewoottacharn K,Sirisuksakun C,Jamnongsong S,Laphanuwat P,Suntiparpluacha M,Matha A,Chusorn P,Buraphat P,Kakanaporn C,Charngkaew K,Silsirivanit A,Korphaisarn K,Limsrichamrern S,Tripatara P,Pairojkul C,Wongkham S,Sampattavanich S,Okada S,Jirawatnotai S
Hepatology. 2019 Nov;70(5):1614-1630

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LOT Information

Cell No.   JCRB1777 Cell Name   KKK-D131
LOT No.   01252019 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   3.0-5.0x10^4 cells/sq.cm Methods for Passages   
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.5x10^6
Viability at cell freezing (%)   95.0 Antibiotics Used   free
Passage Number   p22 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
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