JCRB1779 KKK-D138

Cell information

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About the citation of KKK-series of cell lines.

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1779 Cell Name   KKK-D138
Profile   Human adenosquamous carcinoma cell line established from biliary tract. Other Name   KKK-D138H2
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   60
Identity   not done Tissue for Primary Cancer   liver, gallbladder
Case history    Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   epithelial-like Character   
Classify   tumor Established by   Vaeteewoottacharn, K.
Registered by   Okada, S. Regulation for Distribution   
Comment    Year   2014
Medium   DMEM with 10% fetal bovine serum Methods for Passages   0.25% Trypsin - 0.02% EDTA
Cell Number on Passage   split ratio 1:3 - 1:5 Race   
CO2 Conc.   5% Tissue Sampling   intrahepatic bile duct
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

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Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_XD20
Reference
Pubmed id:31077409Dependency of Cholangiocarcinoma on Cyclin D-Dependent Kinase Activity.
Sittithumcharee G,Suppramote O,Vaeteewoottacharn K,Sirisuksakun C,Jamnongsong S,Laphanuwat P,Suntiparpluacha M,Matha A,Chusorn P,Buraphat P,Kakanaporn C,Charngkaew K,Silsirivanit A,Korphaisarn K,Limsrichamrern S,Tripatara P,Pairojkul C,Wongkham S,Sampattavanich S,Okada S,Jirawatnotai S
Hepatology. 2019 Nov;70(5):1614-1630
Pubmed id:31126020Establishment of Highly Transplantable Cholangiocarcinoma Cell Lines from a Patient-Derived Xenograft Mouse Model.
Vaeteewoottacharn K,Pairojkul C,Kariya R,Muisuk K,Imtawil K,Chamgramol Y,Bhudhisawasdi V,Khuntikeo N,Pugkhem A,Saeseow OT,Silsirivanit A,Wongkham C,Wongkham S,Okada S
Cells. 2019 May 23;8(5):

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LOT Information

Cell No.   JCRB1779 Cell Name   KKK-D138
LOT No.   02192019 Lot Specification   distribution
Medium   DMEM(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   2.0-3.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.02% EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.2x10^6
Viability at cell freezing (%)   91.5 Antibiotics Used   free
Passage Number    PDL   p34
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:9,10
D13S317:11
D7S820:10,11
D16S539:9,11
VWA:14,18
TH01:8,9.3
AM:X
TPOX:11
CSF1PO:10,11
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   Vials are labeled as KKK-D138H2.
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