JCRB1792 KU-Lu-MPPt3

Cell information

Important Notice(s)

KU-Lu-MPPt3の分譲について:営利機関は北里研究所とのMTA締結が必要です

Requirement of MTA between customers and Kitasato Institute for the distribution of KU-Lu-MPPt3 cells to profit-making organizations..

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1792 Cell Name   KU-Lu-MPPt3
Profile   Human lung adenocarcinoma cell line with a micropapillary pattern. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   73-year-old
Identity   not done Tissue for Primary Cancer   lung
Case history   T2aN2M0 Stage IIIA micropapillary adenocarcinoma Metastasis   
Tissue Metastasized    Genetics   EGFR exon19 del E746-A750
Life Span   infinite Crisis PDL   
Morphology    Character   
Classify   tumor Established by   Matsuo, Y.
Registered by   Yukitoshi Sato Regulation for Distribution   営利団体はMTA締結の必要あり
Comment    Year   
Medium   10% FBS RPMI1640, 2mM L-gultamine, N2 supplement. Culture dish/flask should be coated with type I collagen. Methods for Passages   Cells were cultivated with type I collagen coated dish/flask. Cells were harvested after treatment with TrypLE ExPress (Thermo Fisher).
Cell Number on Passage    Race   
CO2 Conc.    Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_QW81
Reference
Pubmed id:29090354 Molecular alterations in a new cell line (KU-Lu-MPPt3) established from a human lung adenocarcinoma with a micropapillary pattern.
Matsuo Y,Shiomi K,Sonoda D,Mikubo M,Naito M,Matsui Y,Yoshida T,Satoh Y
J Cancer Res Clin Oncol. 2018 Jan;144(1):75-87

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LOT Information

Cell No.   JCRB1792 Cell Name   KU-Lu-MPPt3
LOT No.   06142019 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with N-2 supplement(Thermo Fisher) and 10% heat inactivated fetal bovine serum(SIGMA12J396) (cultured by collagen I coated dish) Temperature   37 C
Cell Density at Seeding   1.0-1.3x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with TrypLE ExPress(Thermo Fisher).
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.74x10^6
Viability at cell freezing (%)   89.71 Antibiotics Used   free
Passage Number   p31 PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11
D13S317:11
D7S820:9,12
D16S539:10
VWA:18
TH01:9
AM:X
TPOX:8,11
CSF1PO:9,12
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information   
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