JCRB1801 BALM-25

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1801 Cell Name   BALM-25
Profile   B cell leukemia cell line, ALL-L3 cell line Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   59-year-old
Identity   not done Tissue for Primary Cancer   bone marrow
Case history   Acute lymphoblastic leukemia (ALL) Metastasis   
Tissue Metastasized    Genetics   Karyotype information included in established papers
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   Immnoglobulin phenotype and cell surface marker information are included in established paper.
Classify   tumor Established by   Matsuo Y
Registered by   Fujisaki Cell Center, Hayashibara Biochemical Laboratories, Inc Regulation for Distribution   
Comment    Year   2019
Medium   RPMI1640 medium with 10% featal bovine serum. Methods for Passages   dilution (split ratio 1:2-1:3)/week
Cell Number on Passage    Race   Japanese
CO2 Conc.   5% Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5701
Pubmed id:15160921A novel ALL-L3 cell line, BALM-25, expressing both immunoglobulin light chains.
Matsuo Y,Drexler HG,Harashima A,Takenaka K,Ishihara T,Ishimaru F,Orita K
Leuk Lymphoma. 2004 Mar;45(3):575-81


LOT Information

Cell No.   JCRB1801 Cell Name   BALM-25
LOT No.   07052019 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO) with 10% heat inactivated fetal bovine serum(SIGMA12J396) Temperature   37 C
Cell Density at Seeding   4.0-5.0x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.76x10^6
Viability at cell freezing (%)   92.52 Antibiotics Used   free
Passage Number   p4* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:12,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   NT
Additional information