JCRB1815 MOLM-16

Cell information

Cell type：general cells (View Pricing Information)

JCRB No.   JCRB1815 Cell Name   MOLM-16
Profile   Megakaryoblastic leukemia cell line derived from minimally differentiated acute leukemia (AML-M0) with myeloid/NK precursor phenotype. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   77-year-old
Identity   not done Tissue for Primary Cancer   
Case history   minimally differentiated acute leukemia (AML-M0) Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphoblast-like Character   
Classify   tumor Established by   Matsuo Y
Registered by   Fujisaki Cell Center, Hayashibara Biochemical Laboratories, Inc Regulation for Distribution   
Comment   The survival and growth seem bo be cell density dependent. 1/2 - 1/4 split is recommended. Year   
Medium   RPMI1640 with 10% FBS Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
+/positive.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -
Notes

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_2120
Reference
Pubmed id:12526922Megakaryoblastic leukemia cell line MOLM-16 derived from minimally differentiated acute leukemia with myeloid/NK precursor phenotype.
Matsuo Y,Drexler HG,Kaneda K,Kojima K,Ohtsuki Y,Hara M,Yasukawa M,Tanimoto M,Orita K
Leuk Res. 2003 Feb;27(2):165-71

Images



LOT Information

Cell No.   JCRB1815 Cell Name   MOLM-16
LOT No.   12202019 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO：Cat No.;11875-093) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   0.5-4.0x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.40x10^6
Viability at cell freezing (%)   94.34 Antibiotics Used   free
Passage Number   p8* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:9,12
D7S820:11,12
D16S539:9
VWA:15,18
TH01:9
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   NT Additional information   
Images
Cell No.   JCRB1815 Cell Name   MOLM-16
LOT No.   09062023 Lot Specification   distribution
Medium   RPMI 1640 medium with 10% fetal bovine serum (FBS; GIBCO Cat. # 10437, Lot 1072322) Temperature   37 C
Cell Density at Seeding   1.9 - 2.3 x 10^5 cells/ml Methods for Passages   Simple dilution (suspension culture).
Doubling Time   approx. 50 hrs. Cell Number in Vial (cells/1ml)   3.2 x 10^6
Viability at cell freezing (%)   81 Antibiotics Used   free
Passage Number   Unknown (11 at bank) PDL   
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   
Chromosome Mode    Chromosome Information   
Surface Antigen    DNA Profile (STR)   D5S818:10
D13S317:9,12
D7S820:11,12
D16S539:9
VWA:15,18
TH01:9
AM:X
TPOX:8,11
CSF1PO:12
Adhesion   No Exoteric Gene   
Medium for Freezing   10% DMSO, 20% FBS - RPMI 1640 CO2 Conc.   5%
Viability immediately after thawing (%)   83 Additional information   
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