JCRB1822 NALM-19

Cell information

Important Notice(s)

The cultivation of NALM-19 (NALM-19の培養について)

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1822 Cell Name   NALM-19
Profile   Biphenotypic (B-cell and myeloid cell features) leukemic cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   26-year-old
Identity   not done Tissue for Primary Cancer   peripheral blood
Case history   acute leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   tumor Established by   Matsuo Y
Registered by   Fujisaki Cell Center, Hayashibara Biochemical Laboratories, Inc Regulation for Distribution   
Comment    Year   2019
Medium   RPMI1640 medium + 10% FBS Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   peripheral bood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_1835
Pubmed id:1664237Establishment and characterization of a bi-phenotypic leukemic cell line, NALM-19, from a patient with acute leukemia.
Matsuo Y,Ariyasu T,Adachi T,Tsubota T,Imanishi J,Minowada J
Hum Cell. 1991 Sep;4(3):257-60


LOT Information

Cell No.   JCRB1822 Cell Name   NALM-19
LOT No.   12212020 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   1.94-3.21x10^6 Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   3.15x10^7
Viability at cell freezing (%)   84 Antibiotics Used   free
Passage Number   p12* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   
Adhesion   No Exoteric Gene   NT
Medium for Freezing    CO2 Conc.   5%
Viability immediately after thawing (%)   87 Additional information   The first 1-3weeks after thawing cells do not grow or only very slowly (exchange spent medium wihout dilution).In growth phase,split saturated culture 1:2 every 2-3 days but do not dilute below 2x10^6 cells/ml.