JCRB1822 NALM-19
Cell information
Important Notice(s)The cultivation of NALM-19 (NALM-19の培養について)
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1822 | Cell Name | NALM-19 |
|---|---|---|---|
| Profile | Biphenotypic (B-cell and myeloid cell features) leukemic cell line. | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 26-year-old |
| Identity | not done | Tissue for Primary Cancer | peripheral blood |
| Case history | acute leukemia | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | |
| Classify | tumor | Established by | Matsuo Y |
| Registered by | Fujisaki Cell Center, Hayashibara Biochemical Laboratories, Inc | Regulation for Distribution | |
| Comment | Year | 2019 | |
| Medium | RPMI1640 medium + 10% FBS | Methods for Passages | dilution |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | peripheral bood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:1664237 | Establishment and characterization of a bi-phenotypic leukemic cell line, NALM-19, from a patient with acute leukemia. Matsuo Y,Ariyasu T,Adachi T,Tsubota T,Imanishi J,Minowada J Hum Cell. 1991 Sep;4(3):257-60 |
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LOT Information
| Cell No. | JCRB1822 | Cell Name | NALM-19 |
|---|---|---|---|
| LOT No. | 12212020 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO Cat#11875-093) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) | Temperature | 37 C |
| Cell Density at Seeding | 1.94-3.21x10^6 | Methods for Passages | dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 3.15x10^7 |
| Viability at cell freezing (%) | 84 | Antibiotics Used | free |
| Passage Number | p12* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | CO2 Conc. | 5% | |
| Viability immediately after thawing (%) | 87 | Additional information | The first 1-3weeks after thawing cells do not grow or only very slowly (exchange spent medium wihout dilution).In growth phase,split saturated culture 1:2 every 2-3 days but do not dilute below 2x10^6 cells/ml. |
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