JCRB1828 NALM-25
Cell information
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1828 | Cell Name | NALM-25 |
|---|---|---|---|
| Profile | Biphenotypic (B-cell and myeloid cell features) t(9;22)-positive acute lymphoblastic leukemia cell line. Derived from the same patient as NALM-24 | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 42-year-old |
| Identity | not done | Tissue for Primary Cancer | |
| Case history | acute lymphoblastic leukemia | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | lymphocyte-like | Character | |
| Classify | tumor | Established by | Matsuo Y |
| Registered by | Fujisaki Cell Center, Hayashibara Biochemical Laboratories, Inc | Regulation for Distribution | |
| Comment | Year | 2019 | |
| Medium | RPMI1640 medium +10% FBS | Methods for Passages | dilution |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | peripheral bood |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | not tested | Detected RNA Virus | not tested | ||||||
| Reference | |
|---|---|
| Pubmed id:1667895 | Two t(9;22) leukemia cell lines (NALM-24 and NALM-25) with bi-phenotypic characteristics and an EBV-positive B-cell non-leukemia cell line (B262) established from a patient with acute lymphoblastic leukemia. Matsuo Y,Ariyasu T,Adachi T,Tsubota T,Minowada J Hum Cell. 1991 Dec;4(4):339-42 |
LOT Information
| Cell No. | JCRB1828 | Cell Name | NALM-25 |
|---|---|---|---|
| LOT No. | 09252020 | Lot Specification | distribution |
| Medium | RPMI1640 medium(GIBCO Cat#11875) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) | Temperature | 37 C |
| Cell Density at Seeding | 0.4-5.6x10^5 cells/ml | Methods for Passages | dilution |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.0x10^6 |
| Viability at cell freezing (%) | 91.90 | Antibiotics Used | free |
| Passage Number | p7* | PDL | NT |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10 D13S317:8,10 D7S820:12,13 D16S539:9,11 VWA:14 TH01:7,9 AM:X TPOX:11,12 CSF1PO:10 |
| Adhesion | No | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 79.5 | Additional information | The correct passage number is p7*,wrong number is printed on ampoules. |