JCRB1828 NALM-25

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1828 Cell Name   NALM-25
Profile   Biphenotypic (B-cell and myeloid cell features) t(9;22)-positive acute lymphoblastic leukemia cell line. Derived from the same patient as NALM-24 Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   42-year-old
Identity   not done Tissue for Primary Cancer   
Case history   acute lymphoblastic leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   tumor Established by   Matsuo Y
Registered by   Fujisaki Cell Center, Hayashibara Biochemical Laboratories, Inc Regulation for Distribution   
Comment    Year   2019
Medium   RPMI1640 medium +10% FBS Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   peripheral bood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus not tested Detected RNA Virus not tested

Link
Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_5664
Reference
Pubmed id:1667895Two t(9;22) leukemia cell lines (NALM-24 and NALM-25) with bi-phenotypic characteristics and an EBV-positive B-cell non-leukemia cell line (B262) established from a patient with acute lymphoblastic leukemia.
Matsuo Y,Ariyasu T,Adachi T,Tsubota T,Minowada J
Hum Cell. 1991 Dec;4(4):339-42




LOT Information

Cell No.   JCRB1828 Cell Name   NALM-25
LOT No.   09252020 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO Cat#11875) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012,Lot#12J396) Temperature   37 C
Cell Density at Seeding   0.4-5.6x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.0x10^6
Viability at cell freezing (%)   91.90 Antibiotics Used   free
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10
D13S317:8,10
D7S820:12,13
D16S539:9,11
VWA:14
TH01:7,9
AM:X
TPOX:11,12
CSF1PO:10
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   79.5
Additional information   The correct passage number is p7*,wrong number is printed on ampoules.
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