JCRB1848 TYEK-2
Cell information
Important Notice(s)On the MTA of this cell line with Kyoto University (for profit organizations in Japan)
Announcement of unavailability of this cell line to overseas researcher outside of Japan
Cell type：general cells (View Pricing Information)
| JCRB No. | JCRB1848 | Cell Name | TYEK-2 |
|---|---|---|---|
| Profile | Human squamous cell carcinoma cell line established from laryngeal cancer. (esophageal invasion) | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | M | Age | 75-year-old |
| Identity | not done | Tissue for Primary Cancer | |
| Case history | laryngeal cancer | Metastasis | |
| Tissue Metastasized | Genetics | ||
| Life Span | infinite | Crisis PDL | |
| Morphology | epithelial-like | Character | 1) epithelial morphological features, 2) favorable with collagen coated flasks, 3) doubring time = 21.9 hr (at the time of deposit). 4) avairable for xenograft. 5)T4M1N0 stage 4b |
| Classify | tumor | Established by | Shimada, Y. |
| Registered by | Shimada, Y. | Regulation for Distribution | |
| Comment | Year | ||
| Medium | Ham's F12 medium + RPMI1640 medium (1 to 1 mix) with 5% fetal calf serum. The use of collagen-coated culture vessel to culture is desirable.. | Methods for Passages | Cells harvested after treatment with trypsin and EDTA. split ratio = 1:10 |
| Cell Number on Passage | split ratio 1/10 (DT 21.9h) | Race | |
| CO2 Conc. | 5% | Tissue Sampling | esophagus |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | not tested | ||||||
| CMV | - | parvoB19 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| EBV | - | HBV | - | ||||||
| HHV6 | - | HTLV-1 | - | ||||||
| HHV7 | - | HTLV-2 | - | ||||||
| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
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LOT Information
| Cell No. | JCRB1848 | Cell Name | TYEK-2 |
|---|---|---|---|
| LOT No. | 03022021 | Lot Specification | distribution |
| Medium | RPMI1640(GIBCO Cat#11875-093) and Ham's F12 medium(GIBCO Cat#11765-054) (1/1) with 5% heat inactivated fetal bovine serum(SIGMA,Cat#172012-500ML,Lot#12J396) on collagen I coated dish | Temperature | 37 C |
| Cell Density at Seeding | 2.0-4.0x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 0.01% EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 2.69x10^6 |
| Viability at cell freezing (%) | 99.41 | Antibiotics Used | free |
| Passage Number | PDL | NT | |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10 D13S317:12 D7S820:11 D16S539:9,10 VWA:19 TH01:6,9 AM:X TPOX:8,11 CSF1PO:13 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 80.8 | Additional information | Collagen I coated dish |
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