JCRB1851 Akata Δ2 EBER1(+)

Cell information

Important Notice(s)

JCRB1851 Akata Δ2 EBER1(+)のEBV潜伏感染について

Cell type:virus production cells (View Pricing Information)

JCRB No.   JCRB1851 Cell Name   Akata Δ2 EBER1(+)
Profile   EBV-encoded small RNA (EBER) knockout EBV knocked in EBER1 viruses were reinfected into Akata- cell line. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   4-year-old
Identity   not done Tissue for Primary Cancer   blood
Case history   Burkitt’s lymphoma Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   transformed Established by   Kenzo Takada
Registered by   Kenzo Takada Regulation for Distribution   
Comment    Year   2019
Medium   RPMI1640 with 10% FBS and G418. (Lot check of FBS is important) Methods for Passages   dilution
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   blood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV + HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_B3MK
Pubmed id:17686859Epstein-Barr virus (EBV)-encoded RNA 2 (EBER2) but not EBER1 plays a critical role in EBV-induced B-cell growth transformation.
Wu Y,Maruo S,Yajima M,Kanda T,Takada K
J Virol. 2007 Oct;81(20):11236-45


LOT Information

Cell No.   JCRB1851 Cell Name   Akata Δ2 EBER1(+)
LOT No.   03122021 Lot Specification   distribution
Medium   RPMI1640 medium(GIBCO:Cat No.;11875-093) with 10% heat inactivated fetal bovine serum(SIGMA,Cat#172012-500ML,Lot#12J396) and G418 700 ug/ml Temperature   37 C
Cell Density at Seeding   0.8-2.1x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   2.3x10^6
Viability at cell freezing (%)   93.70 Antibiotics Used   G418
Passage Number   p7* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,12
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   73.9
Additional information