JCRB1136.01 U3-DT

Cell information

Important Notice(s)

POWEREDBY10 medium

Cell type:immortalized mesenchymal stem cell (View Pricing Information)

JCRB No.   JCRB1136.01 Cell Name   U3-DT
Profile   Human bone marrow-derived mesenchymal stem cells (long term culture (PDLs=252)). Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   F Age   91-year-old
Identity   not done Tissue for Primary Cancer   
Case history    Metastasis   No
Tissue Metastasized    Genetics   Human bone marrow-derived mesenchymal stem cells which were infected with recombinant retroviruses expressing the E6, E7, and hTERT.
Life Span   infinite Crisis PDL   
Morphology   fibroblast-like Character   
Classify   transformed Established by   Umezawa, A.
Registered by   Takeuchi, M. Regulation for Distribution   
Comment    Year   2012
Medium   Poweredby10 Methods for Passages   When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells.
Cell Number on Passage    Race   
CO2 Conc.   5% Tissue Sampling   bone marrow
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Cellosaurus(ExPASy) Cellosaurus (ExPASy) is developed by Amos Bairoch of the CALIPHO group at the SIB - Swiss Institute of Bioinformatics as part of the neXtProt project. Cellosaurus is a knowledge database of cell lines with various information summarized. CVCL_B6R9
Pubmed id:25978455Transcriptional Dynamics of Immortalized Human Mesenchymal Stem Cells during Transformation.
Takeuchi M,Higashino A,Takeuchi K,Hori Y,Koshiba-Takeuchi K,Makino H,Monobe Y,Kishida M,Adachi J,Takeuchi J,Tomonaga T,Umezawa A,Kameoka Y,Akagi K
PLoS One. 2015;10(5):e0126562


LOT Information

Cell No.   JCRB1136.01 Cell Name   U3-DT
LOT No.   03262021 Lot Specification   distribution
Medium   POWEREDBY10(GlycoTechnica Ltd.,catalog No.6012 ) Temperature   37 C
Cell Density at Seeding   1.0-5.0x10^4 cells/sq.cm Methods for Passages   Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 1mM EDTA.
Doubling Time   NT Cell Number in Vial (cells/1ml)   1.57x10^6
Viability at cell freezing (%)   98.49 Antibiotics Used   free
Passage Number   p5* PDL   262.2
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:10,13
Adhesion   Yes Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
RFLP    Viability immediately after thawing (%)   63.9
Additional information