JCRB1136.01 U3-DT
Cell information
Important Notice(s)Cell type：immortalized mesenchymal stem cell (View Pricing Information)
| JCRB No. | JCRB1136.01 | Cell Name | U3-DT |
|---|---|---|---|
| Profile | Human bone marrow-derived mesenchymal stem cells (long term culture (PDLs=252)). | Other Name | |
| Animal | human | Strain | |
| Genus | Homo | Species | sapiens |
| Sex | F | Age | 91-year-old |
| Identity | not done | Tissue for Primary Cancer | |
| Case history | Metastasis | No | |
| Tissue Metastasized | Genetics | Human bone marrow-derived mesenchymal stem cells which were infected with recombinant retroviruses expressing the E6, E7, and hTERT. | |
| Life Span | infinite | Crisis PDL | |
| Morphology | fibroblast-like | Character | |
| Classify | transformed | Established by | Umezawa, A. |
| Registered by | Takeuchi, M. | Regulation for Distribution | |
| Comment | Year | 2012 | |
| Medium | Poweredby10 | Methods for Passages | When the cultures reached subconfluence, the cells were harvested with 0.25% trypsin and 1mM EDTA and replated with one-half of harvested cells. |
| Cell Number on Passage | Race | ||
| CO2 Conc. | 5% | Tissue Sampling | bone marrow |
| Tissue Type |
| Detection of virus genome fragment by Real-time PCR | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Detected DNA Virus | tested | Detected RNA Virus | tested | ||||||
| CMV | - | parvoB19 | - | HCV | - | HTLV-1 | - | ||
| EBV | - | HBV | - | HIV-1 | - | HTLV-2 | - | ||
| HHV6 | - | HTLV-1 | - | HIV-2 | - | HAV | - | ||
| HHV7 | - | HTLV-2 | - |
-/negative. +/positive. nt/not tested. (positive (+) does not immediately mean the production of infectious viral particles.) |
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| BKV | - | HIV-1 | - | ||||||
| JCV | - | HIV-2 | - | ||||||
| ADV | - | HPV18 | - | ||||||
| Notes | |||||||||
| Reference | |
|---|---|
| Pubmed id:25978455 | Transcriptional Dynamics of Immortalized Human Mesenchymal Stem Cells during Transformation. Takeuchi M,Higashino A,Takeuchi K,Hori Y,Koshiba-Takeuchi K,Makino H,Monobe Y,Kishida M,Adachi J,Takeuchi J,Tomonaga T,Umezawa A,Kameoka Y,Akagi K PLoS One. 2015;10(5):e0126562 |
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LOT Information
| Cell No. | JCRB1136.01 | Cell Name | U3-DT |
|---|---|---|---|
| LOT No. | 03262021 | Lot Specification | distribution |
| Medium | POWEREDBY10（GlycoTechnica Ltd.,catalog No.6012 ） | Temperature | 37 C |
| Cell Density at Seeding | 1.0-5.0x10^4 cells/sq.cm | Methods for Passages | Cells were harvested after treatment with 0.25%trypsin(GIBCO) and 1mM EDTA. |
| Doubling Time | NT | Cell Number in Vial (cells/1ml) | 1.57x10^6 |
| Viability at cell freezing (%) | 98.49 | Antibiotics Used | free |
| Passage Number | p5* | PDL | 262.2 |
| Sterility: MYCOPLASMA | - | Sterility: BACTERIA | - |
| Sterility: FUNGI | - | Isozyme Analysis | NT |
| Chromosome Mode | NT | Chromosome Information | NT |
| Surface Antigen | NT | DNA Profile (STR) | D5S818:10,13 D13S317:8 D7S820:8,11 D16S539:11 VWA:17,18 TH01:9 AM:X TPOX:9,11 CSF1PO:11,12 |
| Adhesion | Yes | Exoteric Gene | NT |
| Medium for Freezing | BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) | CO2 Conc. | 5% |
| Viability immediately after thawing (%) | 63.9 | Additional information |
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