JCRB1865 NALM-31

Cell information

Cell type:general cells (View Pricing Information)

JCRB No.   JCRB1865 Cell Name   NALM-31
Profile   Biphenotypic features carrying myeloperoxidase mRNA and protein with complex Philadelphia (Ph) chromosome, t(9;22;10)(q34;q11;q22), cell line establish from acute leukemia patient. Derived from the same patient as NALM-27, NALM-28, NALM-30, NALM-32. Other Name   
Animal   human Strain   
Genus   Homo Species   sapiens
Sex   M Age   38-year-old
Identity   not done Tissue for Primary Cancer   peripheral blood
Case history   accute leukemia Metastasis   
Tissue Metastasized    Genetics   
Life Span   infinite Crisis PDL   
Morphology   lymphocyte-like Character   
Classify   tumor Established by   
Registered by   hayashibara Regulation for Distribution   
Comment    Year   2019
Medium   RPMI1640 medium + 10% FBS Methods for Passages   dilution
Cell Number on Passage    Race   Japanese
CO2 Conc.   5% Tissue Sampling   peripheral bood
Tissue Type   

Detection of virus genome fragment by Real-time PCR
Detected DNA Virus tested Detected RNA Virus tested
CMV - parvoB19 - HCV - HTLV-1 -
EBV - HBV - HIV-1 - HTLV-2 -
HHV6 - HTLV-1 - HIV-2 - HAV -
HHV7 - HTLV-2 - -/negative.
nt/not tested.
(positive (+) does not immediately mean the production of infectious viral particles.)
BKV - HIV-1 -
JCV - HIV-2 -
ADV - HPV18 -

Pubmed id:10363160Myeloperoxidase positive acute lymphoblastic leukemia cell lines, NALM-30, NALM-31 and NALM-32, carrying Philadelphia chromosome with biphenotypic characteristics.
Matsuo Y,Sugimoto A,Harashima A,Nishizaki C,Drexler HG,Orita K
Hum Cell. 1998 Dec;11(4):221-30


LOT Information

Cell No.   JCRB1865 Cell Name   NALM-31
LOT No.   08102021 Lot Specification   distribution
Medium   RPMI1640 medium(Wako:catalog No.189-02025) with 10% heat inactivated fetal bovine serum(Biowest cat.No.S1820-500 LotNo.S09336S1820) Temperature   37 C
Cell Density at Seeding   3.16-7.10x10^5 cells/ml Methods for Passages   dilution
Doubling Time   NT Cell Number in Vial (cells/1ml)   4.05x10^6
Viability at cell freezing (%)   87 Antibiotics Used   free
Passage Number   p10* PDL   NT
Sterility: MYCOPLASMA   - Sterility: BACTERIA   -
Sterility: FUNGI   - Isozyme Analysis   NT
Chromosome Mode   NT Chromosome Information   NT
Surface Antigen   NT DNA Profile (STR)   D5S818:11,13
Adhesion   No Exoteric Gene   NT
Medium for Freezing   BAMBANKER(LYMPHOTEC Inc.,CS-02-001,NIPPON Genetics Co., LTD) CO2 Conc.   5%
Viability immediately after thawing (%)   83.3 Additional information